Receptor selectivity of natriuretic peptide family, atrial natriuretic peptide, brain natriuretic peptide, and c-type natriuretic peptide

Shin Ichi Suga, Kazuwa Nakao, Kiminori Hosoda, Masahi Mukoyama, Yoshihiro Ogawa, Gotaro Shirakami, Hiroshi Arai, Yoshihiko Saito, Yoshikazu Kambayashi, Ken Inouye, Hiroo Imura

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628 Citations (Scopus)

Abstract

To elucidate the ligand-receptor relationship of the natriuretic peptide system, which comprises at least three endogenous ligands, atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP), and three receptors, the ANP-A receptor or guanylate cyclase-A (GC-A), the ANP -B receptor or guanylate cyclase-B (GC-B), and the clearance receptor (C-receptor), we characterized the receptor preparations from human, bovine, and rat tissues and cultured cells with the aid of the binding assay, Northern blot technique, and the cGMP production method. Using these receptor preparations, we examined the binding affinities of ANP, BNP, and CNP for the C-receptor and their potencies for cGMP production via the ANP-A receptor (GC-A) and the ANP-B receptor (GC-B). These analyses revealed the presence of a marked species difference in the receptor selectivity of the natriuretic peptide family, especially among BNPs. Therefore, we investigated the receptor selectivity of the natriuretic peptide family using the homologous assay system with endogenous ligands and receptors of the same species. The rank order of binding affinity for the C-receptor was ANP > CNP > BNP in both humans and rats. The rank order of potency for cGMP production via the ANP-A receptor (GC-A) was ANP greater than or equal to BNP ˃˃ CNP, but that via the ANP-B receptor (GC-B) was CNP ˃ ANP greater than or equal to BNP. These findings on the receptor selectivity of the natriuretic peptide family provide a new insight into the understanding of the physiological and clinical implications of the natriuretic peptide system.

Original languageEnglish
Pages (from-to)229-239
Number of pages11
JournalEndocrinology
Volume130
Issue number1
DOIs
Publication statusPublished - Jan 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Endocrinology

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    Suga, S. I., Nakao, K., Hosoda, K., Mukoyama, M., Ogawa, Y., Shirakami, G., Arai, H., Saito, Y., Kambayashi, Y., Inouye, K., & Imura, H. (1992). Receptor selectivity of natriuretic peptide family, atrial natriuretic peptide, brain natriuretic peptide, and c-type natriuretic peptide. Endocrinology, 130(1), 229-239. https://doi.org/10.1210/endo.130.1.1309330