Reciprocal regulation of permeability through a cultured keratinocyte sheet by IFN-γ and IL-4

Junichi Kobayashi, Tetsuichiro Inai, Keisuke Morita, Yoichi Moroi, Kazunori Urabe, Yosaburo Shibata, Masutaka Furue

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The T cell cytokines profoundly modify the phenotypic and functional characteristics of keratinocytes. Until now, no study has focused on the effect of Th1 and Th2 cytokines on keratinocyte permeability. Using a two-layer well culturing system, permeability was assessed through cultured keratinocyte sheet in the presence or absence of various concentrations of IFN-γ and IL-4. Transepithelial electrical resistance (TER) and the flux of 40 kDa FITC-dextrans were measured across the cultured keratinocyte sheet. IFN-γ significantly increased the TER in a dose- and time-dependent manner, suggesting that IFN-γ profoundly inhibited the permeability of ions through the keratinocyte sheet. In contrast, IL-4 did not affect the TER. When compared to medium control, the flux of FITC-dextran of the IFN-γ group was significantly decreased in a dose-dependent fashion. In sharp contrast, the flux of FITC-dextran was significantly and dose-dependently increased in the presence of IL-4. A significant increase in TER and a significant decrease in the flux of dextran suggested that IFN-γ clearly reduced the permeability of both ions and high molecular weight material through the keratinocyte sheet. Although IL-4 did not affect the permeability of the ions, it significantly enhanced the permeability of high molecular weight material. A flow cytometric assay revealed that the expression of desmoglein-3 was suppressed by IL-4, but was enhanced by IFN-γ. The reciprocal regulation of permeability of the cultured keratinocyte sheet by IFN-γ and IL-4 may be partly related to the modification of intercellular adhesion molecules.

Original languageEnglish
Pages (from-to)186-189
Number of pages4
JournalCytokine
Volume28
Issue number4-5
DOIs
Publication statusPublished - Nov 21 2004

Fingerprint

Keratinocytes
Interleukin-4
Permeability
Acoustic impedance
Electric Impedance
Fluxes
Ions
Desmoglein 3
Molecular Weight
Molecular weight
Cytokines
T-cells
Cell Adhesion Molecules
Dextrans
Assays
T-Lymphocytes
fluorescein isothiocyanate dextran

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Reciprocal regulation of permeability through a cultured keratinocyte sheet by IFN-γ and IL-4. / Kobayashi, Junichi; Inai, Tetsuichiro; Morita, Keisuke; Moroi, Yoichi; Urabe, Kazunori; Shibata, Yosaburo; Furue, Masutaka.

In: Cytokine, Vol. 28, No. 4-5, 21.11.2004, p. 186-189.

Research output: Contribution to journalArticle

Kobayashi, J, Inai, T, Morita, K, Moroi, Y, Urabe, K, Shibata, Y & Furue, M 2004, 'Reciprocal regulation of permeability through a cultured keratinocyte sheet by IFN-γ and IL-4', Cytokine, vol. 28, no. 4-5, pp. 186-189. https://doi.org/10.1016/j.cyto.2004.08.003
Kobayashi J, Inai T, Morita K, Moroi Y, Urabe K, Shibata Y et al. Reciprocal regulation of permeability through a cultured keratinocyte sheet by IFN-γ and IL-4. Cytokine. 2004 Nov 21;28(4-5):186-189. https://doi.org/10.1016/j.cyto.2004.08.003
Kobayashi, Junichi ; Inai, Tetsuichiro ; Morita, Keisuke ; Moroi, Yoichi ; Urabe, Kazunori ; Shibata, Yosaburo ; Furue, Masutaka. / Reciprocal regulation of permeability through a cultured keratinocyte sheet by IFN-γ and IL-4. In: Cytokine. 2004 ; Vol. 28, No. 4-5. pp. 186-189.
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