Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain

Saki Inuzuka, Hitoshi Kakizawa, Kei Ichiro Nishimura, Takuto Naito, Katsushi Miyazaki, Hiroyuki Furuta, Shigeyoshi Matsumura, Yoshiya Ikawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The riboswitch is a class of RNA-based gene regulatory machinery that is dependent on recognition of its target ligand by RNA tertiary structures. Ligand recognition is achieved by the aptamer domain, and ligand-dependent structural changes of the expression platform then usually mediate termination of transcription or translational initiation. Ligand-dependent structural changes of the aptamer domain and expression platform have been reported for several riboswitches with short (<40 nucleotides) expression platforms. In this study, we characterized structural changes of the Vc2 c-di-GMP riboswitch that represses translation of downstream open reading frames in a ligand-dependent manner. The Vc2 riboswitch has a long (97 nucleotides) expression platform, but its structure and function are largely unknown. Through mutational analysis and chemical probing, we identified its secondary structures that are possibly responsible for switch-OFF and switch-ON states of translational initiation.

Original languageEnglish
Pages (from-to)435-447
Number of pages13
JournalGenes to Cells
Volume23
Issue number6
DOIs
Publication statusPublished - Jun 2018

Fingerprint

Riboswitch
Ribosomes
Binding Sites
Ligands
Nucleotides
RNA
Regulator Genes
Open Reading Frames
bis(3',5')-cyclic diguanylic acid

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain. / Inuzuka, Saki; Kakizawa, Hitoshi; Nishimura, Kei Ichiro; Naito, Takuto; Miyazaki, Katsushi; Furuta, Hiroyuki; Matsumura, Shigeyoshi; Ikawa, Yoshiya.

In: Genes to Cells, Vol. 23, No. 6, 06.2018, p. 435-447.

Research output: Contribution to journalArticle

Inuzuka, Saki ; Kakizawa, Hitoshi ; Nishimura, Kei Ichiro ; Naito, Takuto ; Miyazaki, Katsushi ; Furuta, Hiroyuki ; Matsumura, Shigeyoshi ; Ikawa, Yoshiya. / Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain. In: Genes to Cells. 2018 ; Vol. 23, No. 6. pp. 435-447.
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