Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum

P. Uparanukraw, T. Harnnoi, A. Dantrakool, N. Morakote, W. Chaicumpa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.

Original languageEnglish
Pages (from-to)273-276
Number of pages4
JournalJournal of Helminthology
Volume73
Issue number3
Publication statusPublished - Jan 1 1999

Fingerprint

gnathostomiasis
Gnathostomiasis
Gnathostoma
antiserum
Immune Sera
monoclonal antibodies
Monoclonal Antibodies
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
glycosidases
Proteins
proteins
somatic antigens
epitopes
Larva
aluminum
glycoproteins
Epitopes
Glycoproteins
peptides
Antigens

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Animal Science and Zoology

Cite this

Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum. / Uparanukraw, P.; Harnnoi, T.; Dantrakool, A.; Morakote, N.; Chaicumpa, W.

In: Journal of Helminthology, Vol. 73, No. 3, 01.01.1999, p. 273-276.

Research output: Contribution to journalArticle

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