Recognition of modification status on a histone H3 tail by linked histone reader modules of the epigenetic regulator UHRF1

Kyohei Arita, Shin Isogai, Takashi Oda, Motoko Unoki, Kazuya Sugita, Naotaka Sekiyama, Keiko Kuwata, Ryuji Hamamoto, Hidehito Tochio, Mamoru Sato, Mariko Ariyoshi, Masahiro Shirakawa

    Research output: Contribution to journalArticle

    88 Citations (Scopus)

    Abstract

    Multiple covalent modifications on a histone tail are often recognized by linked histone reader modules. UHRF1 [ubiquitin-like, containing plant homeodomain (PHD) and really interesting new gene (RING) finger domains 1], an essential factor for maintenance of DNA methylation, contains linked two-histone reader modules, a tandem Tudor domain and a PHD finger, tethered by a 17-aa linker, and has been implicated to link histone modifications and DNA methylation. Here, we present the crystal structure of the linked histone reader modules of UHRF1 in complex with the amino-terminal tail of histone H3. Our structural and biochemical data provide the basis for combinatorial readout of unmodified Arg-2 (H3-R2) and methylated Lys-9 (H3-K9) by the tandem tudor domain and the PHD finger. The structure reveals that the intermodule linker plays an essential role in the formation of a histone H3-binding hole between the reader modules by making extended contacts with the tandem tudor domain. The histone H3 tail fits into the hole by adopting a compact fold harboring a central helix, which allows both of the reader modules to simultaneously recognize the modification states at H3-R2 and H3-K9. Our data also suggest that phosphorylation of a linker residue can modulate the relative position of the reader modules, thereby altering the histone H3-binding mode. This finding implies that the linker region plays a role as a functional switch of UHRF1 involved in multiple regulatory pathways such as maintenance of DNA methylation and transcriptional repression.

    Original languageEnglish
    Pages (from-to)12950-12955
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume109
    Issue number32
    DOIs
    Publication statusPublished - Aug 7 2012

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    Epigenomics
    Histones
    DNA Methylation
    Fingers
    Histone Code
    Maintenance
    Ubiquitin
    Phosphorylation
    Genes
    Tudor Domain

    All Science Journal Classification (ASJC) codes

    • General

    Cite this

    Recognition of modification status on a histone H3 tail by linked histone reader modules of the epigenetic regulator UHRF1. / Arita, Kyohei; Isogai, Shin; Oda, Takashi; Unoki, Motoko; Sugita, Kazuya; Sekiyama, Naotaka; Kuwata, Keiko; Hamamoto, Ryuji; Tochio, Hidehito; Sato, Mamoru; Ariyoshi, Mariko; Shirakawa, Masahiro.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 32, 07.08.2012, p. 12950-12955.

    Research output: Contribution to journalArticle

    Arita, K, Isogai, S, Oda, T, Unoki, M, Sugita, K, Sekiyama, N, Kuwata, K, Hamamoto, R, Tochio, H, Sato, M, Ariyoshi, M & Shirakawa, M 2012, 'Recognition of modification status on a histone H3 tail by linked histone reader modules of the epigenetic regulator UHRF1', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 32, pp. 12950-12955. https://doi.org/10.1073/pnas.1203701109
    Arita, Kyohei ; Isogai, Shin ; Oda, Takashi ; Unoki, Motoko ; Sugita, Kazuya ; Sekiyama, Naotaka ; Kuwata, Keiko ; Hamamoto, Ryuji ; Tochio, Hidehito ; Sato, Mamoru ; Ariyoshi, Mariko ; Shirakawa, Masahiro. / Recognition of modification status on a histone H3 tail by linked histone reader modules of the epigenetic regulator UHRF1. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 32. pp. 12950-12955.
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