TY - JOUR
T1 - Recombinant carp IL-4/13B stimulates in vitro proliferation of carp IgM+ B cells
AU - Yamaguchi, Takuya
AU - Miyata, Shunsuke
AU - Katakura, Fumihiko
AU - Nagasawa, Takahiro
AU - Shibasaki, Yasuhiro
AU - Yabu, Takeshi
AU - Fischer, Uwe
AU - Nakayasu, Chihaya
AU - Nakanishi, Teruyuki
AU - Moritomo, Tadaaki
N1 - Funding Information:
We thank Dr. Johannes M. Dijkstra (Institute for Comprehensive Medical Science, Fujita Health University, Japan) for helpful suggestions. This work was supported by the EU FP7 grant 311993 (TargetFish) for TY and UF.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Teleost IL-4/13B is a cytokine related to mammalian IL-4 and IL-13, of which hitherto the function had not been studied at the protein level. We identified an IL-4/13B gene in common carp (Cyprinus carpio) and expressed the recombinant protein (rcIL-4/13B). RcIL-4/13B was shown to stimulate proliferation of IgM+ B cells, because after four days of stimulation the IgM+ fraction of carp kidney and spleen leukocytes had formed many cell colonies, whereas such colonies were not found in the absence of rcIL-4/13B stimulation. After nine days of incubation with rcIL-4/13B these cells had proliferated to more than 3-to-7-fold higher numbers when compared to untreated cells. The proliferating cells contained a majority of IgM+ cells but also other cells, as indicated by FACS and RT-PCR analyses. The important conclusion is that in fish not only IL-4/13A has B cell stimulating properties, as a previous publication has shown, but also IL-4/13B.
AB - Teleost IL-4/13B is a cytokine related to mammalian IL-4 and IL-13, of which hitherto the function had not been studied at the protein level. We identified an IL-4/13B gene in common carp (Cyprinus carpio) and expressed the recombinant protein (rcIL-4/13B). RcIL-4/13B was shown to stimulate proliferation of IgM+ B cells, because after four days of stimulation the IgM+ fraction of carp kidney and spleen leukocytes had formed many cell colonies, whereas such colonies were not found in the absence of rcIL-4/13B stimulation. After nine days of incubation with rcIL-4/13B these cells had proliferated to more than 3-to-7-fold higher numbers when compared to untreated cells. The proliferating cells contained a majority of IgM+ cells but also other cells, as indicated by FACS and RT-PCR analyses. The important conclusion is that in fish not only IL-4/13A has B cell stimulating properties, as a previous publication has shown, but also IL-4/13B.
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U2 - 10.1016/j.fsi.2015.12.043
DO - 10.1016/j.fsi.2015.12.043
M3 - Article
C2 - 26766176
AN - SCOPUS:84952909060
SN - 1050-4648
VL - 49
SP - 225
EP - 229
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
ER -