Recombinant expression of perchloric acid-soluble protein reduces cell proliferation

H. Kanouchi, Hirofumi Tachibana, T. Oka, K. Yamada

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Perchloric acid-soluble protein (PSP) may play an important role in the regulation of cellular physiological functions because it has been highly conserved throughout evolution; however, this role has not been well elucidated. In previous reports, we suggested that PSP regulates cell proliferation. In this study, we examined the effect of PSP expression on proliferation of the normal rat kidney cell line NRK-52E, the rat hepatocyte cell line RLN-10, and the rat hepatoma cell line dRLh-84. Cells transfected with pcDNA-sense-PSP (pcDNA-S-PSP) over-expressed PSP mRNA and protein, and cell proliferation of the transfected cells was suppressed compared with that of cells transfected with pcDNA-empty (pcDNA-E). Cell viability of pcDNA-S-PSP-transfected cells was similar to that of pcDNA-E-transfected cells. Thus, over-expression of PSP suppresses cell proliferation without any influence on cell viability. These findings are the first to report an inhibitory activity of PSP on cell proliferation.

Original languageEnglish
Pages (from-to)1340-1343
Number of pages4
JournalCellular and Molecular Life Sciences
Volume58
Issue number9
DOIs
Publication statusPublished - Jan 1 2001

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Cell Proliferation
Proteins
Cell Line
Cell Survival
Perchloric Acid
Hepatocytes
Hepatocellular Carcinoma
Kidney
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Recombinant expression of perchloric acid-soluble protein reduces cell proliferation. / Kanouchi, H.; Tachibana, Hirofumi; Oka, T.; Yamada, K.

In: Cellular and Molecular Life Sciences, Vol. 58, No. 9, 01.01.2001, p. 1340-1343.

Research output: Contribution to journalArticle

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