Recombinant mitochondrial transcription factor A protein inhibits nuclear factor of activated T cells signaling and attenuates pathological hypertrophy of cardiac myocytes

Takeo Fujino, Tomomi Ide, Masayoshi Yoshida, Ken Onitsuka, Atsushi Tanaka, Yuko Hata, Motohiro Nishida, Takako Takehara, Takaaki Kanemaru, Naoyuki Kitajima, Shinya Takazaki, Hitoshi Kurose, Dongchon Kang, Kenji Sunagawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The overexpression of mitochondrial transcription factor A (TFAM) attenuates the decrease in mtDNA copy number after myocardial infarction, ameliorates pathological hypertrophy, and markedly improves survival. However, non-transgenic strategy to increase mtDNA for the treatment of pathological hypertrophy remains unknown. We produced recombinant human TFAM protein (rhTFAM). rhTFAM rapidly entered into mitochondria of cultured cardiac myocytes. rhTFAM increased mtDNA and abolished the activation of nuclear factor of activated T cells (NFAT), which is well known to activate pathological hypertrophy. rhTFAM attenuated subsequent morphological hypertrophy of myocytes as well. rhTFAM would be an attractive molecule in attenuating cardiac pathological hypertrophy.

Original languageEnglish
Pages (from-to)449-458
Number of pages10
JournalMitochondrion
Volume12
Issue number4
DOIs
Publication statusPublished - Jul 1 2012

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NFATC Transcription Factors
Cardiac Myocytes
Hypertrophy
Mitochondrial DNA
Proteins
Cardiomegaly
Muscle Cells
Mitochondria
Myocardial Infarction
mitochondrial transcription factor A
human TFAM protein

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Recombinant mitochondrial transcription factor A protein inhibits nuclear factor of activated T cells signaling and attenuates pathological hypertrophy of cardiac myocytes. / Fujino, Takeo; Ide, Tomomi; Yoshida, Masayoshi; Onitsuka, Ken; Tanaka, Atsushi; Hata, Yuko; Nishida, Motohiro; Takehara, Takako; Kanemaru, Takaaki; Kitajima, Naoyuki; Takazaki, Shinya; Kurose, Hitoshi; Kang, Dongchon; Sunagawa, Kenji.

In: Mitochondrion, Vol. 12, No. 4, 01.07.2012, p. 449-458.

Research output: Contribution to journalArticle

Fujino, Takeo ; Ide, Tomomi ; Yoshida, Masayoshi ; Onitsuka, Ken ; Tanaka, Atsushi ; Hata, Yuko ; Nishida, Motohiro ; Takehara, Takako ; Kanemaru, Takaaki ; Kitajima, Naoyuki ; Takazaki, Shinya ; Kurose, Hitoshi ; Kang, Dongchon ; Sunagawa, Kenji. / Recombinant mitochondrial transcription factor A protein inhibits nuclear factor of activated T cells signaling and attenuates pathological hypertrophy of cardiac myocytes. In: Mitochondrion. 2012 ; Vol. 12, No. 4. pp. 449-458.
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AU - Hata, Yuko

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