TY - JOUR
T1 - Recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing Ag85B-IL-7 fusion protein enhances IL-17A-producing innate γδ T cells
AU - Hatano, Shinya
AU - Tamura, Toshiki
AU - Umemura, Masayuki
AU - Matsuzaki, Goro
AU - Ohara, Naoya
AU - Yoshikai, Yasunobu
N1 - Funding Information:
This work was supported by Grants-in-Aid ( 25670213 ; 26293098 ) from the Japan Society for the Promotion of Science and the Takeda Science Foundation (Y.Y.). We thank, Miki Kijima and Akiko Yano for secretarial assistance, and Dr. K. Ikuta for providing mice.
Publisher Copyright:
© 2016 Elsevier Ltd.
PY - 2016/5/11
Y1 - 2016/5/11
N2 - Interleukin 7 (IL-7) has an important function in the development and maintenance of IL-17A+ γδ T cells. We here constructed a recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing antigen 85B (Ag85B)-IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacterial lysate of rBCG-Ag85B-IL-7. rBCG-Ag85B-IL-7 was the same in number as control rBCG expressing Ag85B (rBCG-Ag85B) in the lung at the early stage after intravenous inoculation, whereas the numbers of IL-17A+ γδ T cells and Ag-specific Th1 cells were significantly higher in the lungs of mice inoculated with rBCG-Ag85B-IL-7 than those inoculated with rBCG-Ag85B. The Ag-specific Th1 cell response was impaired in mice lacking IL-17A+ γδ T cells after inoculation with rBCG-Ag85B-IL-7. Thus, rBCG-Ag85B-IL-7 increases the pool size of IL-17A+ γδ T cells, which subsequently augment the Th1 response to mycobacterial infection.
AB - Interleukin 7 (IL-7) has an important function in the development and maintenance of IL-17A+ γδ T cells. We here constructed a recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing antigen 85B (Ag85B)-IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacterial lysate of rBCG-Ag85B-IL-7. rBCG-Ag85B-IL-7 was the same in number as control rBCG expressing Ag85B (rBCG-Ag85B) in the lung at the early stage after intravenous inoculation, whereas the numbers of IL-17A+ γδ T cells and Ag-specific Th1 cells were significantly higher in the lungs of mice inoculated with rBCG-Ag85B-IL-7 than those inoculated with rBCG-Ag85B. The Ag-specific Th1 cell response was impaired in mice lacking IL-17A+ γδ T cells after inoculation with rBCG-Ag85B-IL-7. Thus, rBCG-Ag85B-IL-7 increases the pool size of IL-17A+ γδ T cells, which subsequently augment the Th1 response to mycobacterial infection.
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U2 - 10.1016/j.vaccine.2016.03.096
DO - 10.1016/j.vaccine.2016.03.096
M3 - Article
C2 - 27079930
AN - SCOPUS:84963526801
SN - 0264-410X
VL - 34
SP - 2490
EP - 2495
JO - Vaccine
JF - Vaccine
IS - 22
ER -
