Recombination between gtfB and gtfC is required for survival of a dTDP- rhamnose synthesis-deficient mutant of Streptococcus mutans in the presence of sucrose

Yoshihisa Yamashita, Kiyotaka Tomihisa, Yoshio Nakano, Yoshihiro Shimazaki, Takahiko Oho, Toshihiko Koga

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The rml genes are involved in dTDP-rhamnose synthesis in Streptococcus mutans. A gene fusion between gtfB and gtfC, which both encode extracellular water-insoluble glucan-synthesizing enzymes, accompanied by inactivation of the rml genes was observed for cells grown in the presence of sucrose. The survival rates of rml mutants isolated in the absence of sucrose were drastically reduced in the presence of sucrose. The rates were consistent with the frequency of spontaneous gene fusions between gtfB and gtfC, suggesting that the spontaneous recombinant organisms were selected in the presence of sucrose. The rml mutants with a gtfB-gtfC fusion gene had markedly reduced water-insoluble glucan synthetic activity and lost the ability to colonize glass surfaces in the presence of sucrose. These results suggest that the rml mutants of S. mutans, which are defective in dTDP- rhamnose synthesis, can survive only in the absence of water-insoluble glucan synthesis.

Original languageEnglish
Pages (from-to)3693-3697
Number of pages5
JournalInfection and Immunity
Volume67
Issue number7
Publication statusPublished - 1999

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Rhamnose
Streptococcus mutans
Genetic Recombination
Sucrose
Glucans
Gene Fusion
Water
Gene Silencing
Glass
Enzymes
Genes

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Recombination between gtfB and gtfC is required for survival of a dTDP- rhamnose synthesis-deficient mutant of Streptococcus mutans in the presence of sucrose. / Yamashita, Yoshihisa; Tomihisa, Kiyotaka; Nakano, Yoshio; Shimazaki, Yoshihiro; Oho, Takahiko; Koga, Toshihiko.

In: Infection and Immunity, Vol. 67, No. 7, 1999, p. 3693-3697.

Research output: Contribution to journalArticle

Yamashita, Yoshihisa ; Tomihisa, Kiyotaka ; Nakano, Yoshio ; Shimazaki, Yoshihiro ; Oho, Takahiko ; Koga, Toshihiko. / Recombination between gtfB and gtfC is required for survival of a dTDP- rhamnose synthesis-deficient mutant of Streptococcus mutans in the presence of sucrose. In: Infection and Immunity. 1999 ; Vol. 67, No. 7. pp. 3693-3697.
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abstract = "The rml genes are involved in dTDP-rhamnose synthesis in Streptococcus mutans. A gene fusion between gtfB and gtfC, which both encode extracellular water-insoluble glucan-synthesizing enzymes, accompanied by inactivation of the rml genes was observed for cells grown in the presence of sucrose. The survival rates of rml mutants isolated in the absence of sucrose were drastically reduced in the presence of sucrose. The rates were consistent with the frequency of spontaneous gene fusions between gtfB and gtfC, suggesting that the spontaneous recombinant organisms were selected in the presence of sucrose. The rml mutants with a gtfB-gtfC fusion gene had markedly reduced water-insoluble glucan synthetic activity and lost the ability to colonize glass surfaces in the presence of sucrose. These results suggest that the rml mutants of S. mutans, which are defective in dTDP- rhamnose synthesis, can survive only in the absence of water-insoluble glucan synthesis.",
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AU - Tomihisa, Kiyotaka

AU - Nakano, Yoshio

AU - Shimazaki, Yoshihiro

AU - Oho, Takahiko

AU - Koga, Toshihiko

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AB - The rml genes are involved in dTDP-rhamnose synthesis in Streptococcus mutans. A gene fusion between gtfB and gtfC, which both encode extracellular water-insoluble glucan-synthesizing enzymes, accompanied by inactivation of the rml genes was observed for cells grown in the presence of sucrose. The survival rates of rml mutants isolated in the absence of sucrose were drastically reduced in the presence of sucrose. The rates were consistent with the frequency of spontaneous gene fusions between gtfB and gtfC, suggesting that the spontaneous recombinant organisms were selected in the presence of sucrose. The rml mutants with a gtfB-gtfC fusion gene had markedly reduced water-insoluble glucan synthetic activity and lost the ability to colonize glass surfaces in the presence of sucrose. These results suggest that the rml mutants of S. mutans, which are defective in dTDP- rhamnose synthesis, can survive only in the absence of water-insoluble glucan synthesis.

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