Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer

Pamela S. Ohashi, Tak W. Mak, Peter Van Den Elsen, Yusuke Yanagi, Yasunobu Yoshikai, Andrew F. Calman, Cox Terhorst, John D. Stobo, Arthur Weiss

    Research output: Contribution to journalArticle

    187 Citations (Scopus)

    Abstract

    We have introduced a full-length complementary DNA of the T-cell antigen receptor β-chain into a mutant human T-cell line that lacked a complete β-chain messenger RNA, had a diminished level of α-chain transcript and did not express surface T3- or antigen receptor. Expression of the transfected β-chain led to a normal level of α-chain transcript and a structurally and functionally active T3 T-cell antigen receptor complex on the cell surface.

    Original languageEnglish
    Pages (from-to)606-609
    Number of pages4
    JournalNature
    Volume316
    Issue number6029
    DOIs
    Publication statusPublished - Dec 1 1985

    Fingerprint

    CD3 Antigens
    T-Cell Antigen Receptor
    Thyroid Hormone Receptors
    Antigen Receptors
    DNA
    Surface Antigens
    Complementary DNA
    T-Lymphocytes
    Cell Line
    Messenger RNA

    All Science Journal Classification (ASJC) codes

    • Medicine(all)
    • General

    Cite this

    Ohashi, P. S., Mak, T. W., Van Den Elsen, P., Yanagi, Y., Yoshikai, Y., Calman, A. F., ... Weiss, A. (1985). Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer. Nature, 316(6029), 606-609. https://doi.org/10.1038/316606a0

    Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer. / Ohashi, Pamela S.; Mak, Tak W.; Van Den Elsen, Peter; Yanagi, Yusuke; Yoshikai, Yasunobu; Calman, Andrew F.; Terhorst, Cox; Stobo, John D.; Weiss, Arthur.

    In: Nature, Vol. 316, No. 6029, 01.12.1985, p. 606-609.

    Research output: Contribution to journalArticle

    Ohashi, PS, Mak, TW, Van Den Elsen, P, Yanagi, Y, Yoshikai, Y, Calman, AF, Terhorst, C, Stobo, JD & Weiss, A 1985, 'Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer', Nature, vol. 316, no. 6029, pp. 606-609. https://doi.org/10.1038/316606a0
    Ohashi PS, Mak TW, Van Den Elsen P, Yanagi Y, Yoshikai Y, Calman AF et al. Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer. Nature. 1985 Dec 1;316(6029):606-609. https://doi.org/10.1038/316606a0
    Ohashi, Pamela S. ; Mak, Tak W. ; Van Den Elsen, Peter ; Yanagi, Yusuke ; Yoshikai, Yasunobu ; Calman, Andrew F. ; Terhorst, Cox ; Stobo, John D. ; Weiss, Arthur. / Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer. In: Nature. 1985 ; Vol. 316, No. 6029. pp. 606-609.
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    AU - Yoshikai, Yasunobu

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    AU - Terhorst, Cox

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