Reconstruction of cartilage tissue using scaffold-free organoid culture technique

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We applied the scaffold-free culture method to chondrocytes and attempted to reconstitute articular cartilage grafts. Primary rat costal chondrocytes were immobilized into hollow fibers by centrifugation at a density of 3×108 cells/cm3 to induce the formation of cylindrical-shaped multicellular aggregates (organoids) and cultured for one month. The organoids were evaluated by histological and gene expression analyses. Chondrocytes formed cylindrical organoids in hollow fibers (HFs). Histochemical analysis revealed the accumulation of a cartilage extracellular matrix (collagen and proteoglycan) around cells in the lumen of HFs with culture time, forming a low-cellular-density tissue similar to native cartilage by day 28. Furthermore, in contrast to that in traditional monolayer culture, the organoid maintained the gene expression of the cartilage extracellular matrix (type II collagen, aggrecan) for one month of culture. In conclusion, our organoid formation method was effective in producing a cartilage-like tissue. This result suggests that the technique may be applicable to the development of an articular cartilage graft.

Original languageEnglish
Pages (from-to)450-453
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume105
Issue number5
DOIs
Publication statusPublished - May 1 2008

Fingerprint

Organoids
Tissue Scaffolds
Culture Techniques
Cartilage
Chondrocytes
Articular Cartilage
Extracellular Matrix
Collagen
Gene expression
Grafts
Fibers
Transplants
Gene Expression
Aggrecans
Collagen Type II
Tissue
Proteoglycans
Centrifugation
Scaffolds
Cell Count

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering

Cite this

@article{3090babb13ec47339f8590d0a70d2b7c,
title = "Reconstruction of cartilage tissue using scaffold-free organoid culture technique",
abstract = "We applied the scaffold-free culture method to chondrocytes and attempted to reconstitute articular cartilage grafts. Primary rat costal chondrocytes were immobilized into hollow fibers by centrifugation at a density of 3×108 cells/cm3 to induce the formation of cylindrical-shaped multicellular aggregates (organoids) and cultured for one month. The organoids were evaluated by histological and gene expression analyses. Chondrocytes formed cylindrical organoids in hollow fibers (HFs). Histochemical analysis revealed the accumulation of a cartilage extracellular matrix (collagen and proteoglycan) around cells in the lumen of HFs with culture time, forming a low-cellular-density tissue similar to native cartilage by day 28. Furthermore, in contrast to that in traditional monolayer culture, the organoid maintained the gene expression of the cartilage extracellular matrix (type II collagen, aggrecan) for one month of culture. In conclusion, our organoid formation method was effective in producing a cartilage-like tissue. This result suggests that the technique may be applicable to the development of an articular cartilage graft.",
author = "Yutaka Irie and Hiroshi Mizumoto and Shigeru Fujino and Toshihisa Kajiwara",
year = "2008",
month = "5",
day = "1",
doi = "10.1263/jbb.105.450",
language = "English",
volume = "105",
pages = "450--453",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "5",

}

TY - JOUR

T1 - Reconstruction of cartilage tissue using scaffold-free organoid culture technique

AU - Irie, Yutaka

AU - Mizumoto, Hiroshi

AU - Fujino, Shigeru

AU - Kajiwara, Toshihisa

PY - 2008/5/1

Y1 - 2008/5/1

N2 - We applied the scaffold-free culture method to chondrocytes and attempted to reconstitute articular cartilage grafts. Primary rat costal chondrocytes were immobilized into hollow fibers by centrifugation at a density of 3×108 cells/cm3 to induce the formation of cylindrical-shaped multicellular aggregates (organoids) and cultured for one month. The organoids were evaluated by histological and gene expression analyses. Chondrocytes formed cylindrical organoids in hollow fibers (HFs). Histochemical analysis revealed the accumulation of a cartilage extracellular matrix (collagen and proteoglycan) around cells in the lumen of HFs with culture time, forming a low-cellular-density tissue similar to native cartilage by day 28. Furthermore, in contrast to that in traditional monolayer culture, the organoid maintained the gene expression of the cartilage extracellular matrix (type II collagen, aggrecan) for one month of culture. In conclusion, our organoid formation method was effective in producing a cartilage-like tissue. This result suggests that the technique may be applicable to the development of an articular cartilage graft.

AB - We applied the scaffold-free culture method to chondrocytes and attempted to reconstitute articular cartilage grafts. Primary rat costal chondrocytes were immobilized into hollow fibers by centrifugation at a density of 3×108 cells/cm3 to induce the formation of cylindrical-shaped multicellular aggregates (organoids) and cultured for one month. The organoids were evaluated by histological and gene expression analyses. Chondrocytes formed cylindrical organoids in hollow fibers (HFs). Histochemical analysis revealed the accumulation of a cartilage extracellular matrix (collagen and proteoglycan) around cells in the lumen of HFs with culture time, forming a low-cellular-density tissue similar to native cartilage by day 28. Furthermore, in contrast to that in traditional monolayer culture, the organoid maintained the gene expression of the cartilage extracellular matrix (type II collagen, aggrecan) for one month of culture. In conclusion, our organoid formation method was effective in producing a cartilage-like tissue. This result suggests that the technique may be applicable to the development of an articular cartilage graft.

UR - http://www.scopus.com/inward/record.url?scp=44949142146&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44949142146&partnerID=8YFLogxK

U2 - 10.1263/jbb.105.450

DO - 10.1263/jbb.105.450

M3 - Article

C2 - 18558333

AN - SCOPUS:44949142146

VL - 105

SP - 450

EP - 453

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 5

ER -