Recovery of two independent sweet taste systems during regeneration of the mouse chorda tympani nerve after nerve crush

Keiko Nakano, Yoko Kusuhara, Noriatsu Shigemura, Yuzo Ninomiya

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In rodents, section of the taste nerve results in degeneration of the taste buds. Following regeneration of the cut taste nerve, however, the taste buds reappear. This phenomenon can be used to study the functional reformation of the peripheral neural system responsible for sweet taste. In this study we examined the recovery of sweet responses by the chorda tympani (CT) nerve after nerve crush as well as inhibition of these responses by gurmarin (Gur), a sweet response inhibitor. After about 2 weeks of CT nerve regeneration, no significant response to any taste stimuli could be observed. At 3 weeks, responses to sweet stimuli reappeared but were not significantly inhibited by Gur. At 4 weeks, Gur inhibition of sweet responses reached statistically significant levels. Thus, the Gur-sensitive (GS) component of the sweet response reappeared about 1 week later than the Gur-insensitive (GI) component. Moreover, single CT fibers responsive to sucrose could be classified into distinct GS and GI groups at 4 weeks. After 5 weeks or more, responses to sweet compounds before and after treatment with Gur became indistinguishable from responses in the intact group. During regeneration, the GS and GI components of the sucrose response could be distinguished based on their concentration-dependent responses to sucrose. These results suggest that mice have two different sweet-reception systems, distinguishable by their sensitivity to Gur (the GS and GI systems). These two sweet-reception systems may be reconstituted independently during regeneration of the mouse CT nerve.

Original languageEnglish
Pages (from-to)1521-1529
Number of pages9
JournalEuropean Journal of Neuroscience
Volume26
Issue number6
DOIs
Publication statusPublished - Sep 1 2007

Fingerprint

Chorda Tympani Nerve
Nerve Crush
Regeneration
Taste Buds
Sucrose
Nerve Regeneration
Gymnema sylvestre Gurmarin protein
Gur
Rodentia

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Recovery of two independent sweet taste systems during regeneration of the mouse chorda tympani nerve after nerve crush. / Nakano, Keiko; Kusuhara, Yoko; Shigemura, Noriatsu; Ninomiya, Yuzo.

In: European Journal of Neuroscience, Vol. 26, No. 6, 01.09.2007, p. 1521-1529.

Research output: Contribution to journalArticle

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