Red-shifted red/green-type cyanobacteriochrome AM1-1870g3 from the chlorophyll d-bearing cyanobacterium Acaryochloris marina

Rei Narikawa, Keiji Fushimi, Ni-Ni-Win, Masahiko Ikeuchi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Cyanobacteriochromes (CBCRs) are diverse photoreceptors that are found only from cyanobacteria and cover wide range of light qualities. CBCRs are divided into two types regarding the chromophore species they contain: phycocyanobilin (PCB) and phycoviolobilin. Red/green-type CBCRs are widely distributed subfamily among the PCB-binding CBCRs and photoconvert between a red-absorbing thermostable form and a green-absorbing metastable form. Our recent study discovered that a red/green-type CBCR, AM1-1557g2, from a cyanobacterium Acaryochloris marina covalently binds not only PCB but also biliverdin (BV). BV-binding AM1-1557g2 photoconverts between a far-red absorbing form and an orange-absorbing form. We report, herein, that another red/green-type CBCR, AM1-1870g3, from the cyanobacterium A. marina also bound both PCB and BV. PCB- and BV-binding ones showed red/green and far-red/orange reversible photoconversions, respectively. Unexpectedly, absorbing wavelengths are 10-20 nm red-shifted compared with those of AM1-1557g2. These red-shifted characteristics may be useful for optogenetic light switches that work in various organisms.

Original languageEnglish
Pages (from-to)390-395
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume461
Issue number2
DOIs
Publication statusPublished - May 8 2015
Externally publishedYes

Fingerprint

Bearings (structural)
Marinas
Biliverdine
Cyanobacteria
Optogenetics
Light
Chromophores
Switches
phycocyanobilin
chlorophyll d
Wavelength

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Red-shifted red/green-type cyanobacteriochrome AM1-1870g3 from the chlorophyll d-bearing cyanobacterium Acaryochloris marina. / Narikawa, Rei; Fushimi, Keiji; Ni-Ni-Win; Ikeuchi, Masahiko.

In: Biochemical and Biophysical Research Communications, Vol. 461, No. 2, 08.05.2015, p. 390-395.

Research output: Contribution to journalArticle

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