Reduced expression of the dysbindin -like gene in the Bombyx mori ov mutant exhibiting mottled translucency of the larval skin

Lingyan Wang, Takashi Kiuchi, Tsuguru Fujii, Takaaki Daimon, Muwang Li, Yutaka Banno, Susumu Katsuma, Toru Shimada

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The ov (mottled translucent of Var) mutant, an oily mutant of Bombyx mori, exhibits mottled translucent skin with a varying degree of transparency among individuals. By linkage analysis of 2112 backcross individuals using polymorphic DNA markers, we successfully mapped a 179-kb region of chromosome 20 responsible for the ov phenotype. This region contains nine predicted genes. We compared the mRNA expression of these nine genes between the wild type and mutants and found that the expression of one of them, Bmdysb, was strikingly decreased in the epidermis of ov as well as its allelomorph, ovp. Moreover, its expression level was well correlated with the degree of transparency among individuals. Bmdysb was homologous to DTNBP1 encoding human dysbindin, a subunit of the biogenesis of lysosome-related organelles complex-1. Our results suggest that the translucent skin may be due to repression of Bmdysb in the ov mutants and that Bmdysb plays an important role in the formation and accumulation of urate granules in the silkworm epidermis.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalGenome
Volume56
Issue number2
DOIs
Publication statusPublished - Feb 2013

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Genetics

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