Reduced motor and sensory functions and emotional response in GM3-only mice: Emergence from early stage of life and exacerbation with aging

Orie Tajima, Nobuaki Egashira, Yuhsuke Ohmi, Yoshihiko Fukue, Kenichi Mishima, Katsunori Iwasaki, Michihiro Fujiwara, Jinichi Inokuchi, Yasuo Sugiura, Keiko Furukawa, Koichi Furukawa

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Sialic acid-containing glycosphingolipids (gangliosides) have been believed to play a role in the regulation and protection of nervous tissues. To clarify their function in the nervous system in vivo, double knockout (DKO) mice of GM2/GD2 synthase and GD3 synthase genes were generated and abnormal behaviors were analyzed. Mutant mice exhibited reduced weight and a round shape of the whole brain that progressively emerged with aging, and displayed motor dysfunction in the footprint, traction, open-field, and 24 h locomotion activity tests. Sensory functions were also reduced in the von Frey and hot plate tests and greatly reduced in the acoustic startle response test. For emotional behavior, fear response was clearly decreased. Numerous neuronal dysfunctions were found even in younger mutant mice examined at 10-23 weeks after birth, which were exacerbated with aging. These results suggest that a lack of gangliosides other than GM3 induces severe neuronal degeneration in the early stage of life, and that the expression of complex gangliosides is essential to maintain the integrity of the nervous system throughout life.

Original languageEnglish
Pages (from-to)74-82
Number of pages9
JournalBehavioural Brain Research
Volume198
Issue number1
DOIs
Publication statusPublished - Mar 2 2009

Fingerprint

(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase
Gangliosides
Nervous System
Startle Reflex
G(M3) Ganglioside
Nerve Tissue
Glycosphingolipids
Traction
N-Acetylneuraminic Acid
Locomotion
Acoustics
Knockout Mice
Fear
Parturition
Weights and Measures
Brain
Genes

All Science Journal Classification (ASJC) codes

  • Behavioral Neuroscience

Cite this

Reduced motor and sensory functions and emotional response in GM3-only mice : Emergence from early stage of life and exacerbation with aging. / Tajima, Orie; Egashira, Nobuaki; Ohmi, Yuhsuke; Fukue, Yoshihiko; Mishima, Kenichi; Iwasaki, Katsunori; Fujiwara, Michihiro; Inokuchi, Jinichi; Sugiura, Yasuo; Furukawa, Keiko; Furukawa, Koichi.

In: Behavioural Brain Research, Vol. 198, No. 1, 02.03.2009, p. 74-82.

Research output: Contribution to journalArticle

Tajima, O, Egashira, N, Ohmi, Y, Fukue, Y, Mishima, K, Iwasaki, K, Fujiwara, M, Inokuchi, J, Sugiura, Y, Furukawa, K & Furukawa, K 2009, 'Reduced motor and sensory functions and emotional response in GM3-only mice: Emergence from early stage of life and exacerbation with aging', Behavioural Brain Research, vol. 198, no. 1, pp. 74-82. https://doi.org/10.1016/j.bbr.2008.10.024
Tajima, Orie ; Egashira, Nobuaki ; Ohmi, Yuhsuke ; Fukue, Yoshihiko ; Mishima, Kenichi ; Iwasaki, Katsunori ; Fujiwara, Michihiro ; Inokuchi, Jinichi ; Sugiura, Yasuo ; Furukawa, Keiko ; Furukawa, Koichi. / Reduced motor and sensory functions and emotional response in GM3-only mice : Emergence from early stage of life and exacerbation with aging. In: Behavioural Brain Research. 2009 ; Vol. 198, No. 1. pp. 74-82.
@article{e8dc20b135e943359ecdbe9386d70588,
title = "Reduced motor and sensory functions and emotional response in GM3-only mice: Emergence from early stage of life and exacerbation with aging",
abstract = "Sialic acid-containing glycosphingolipids (gangliosides) have been believed to play a role in the regulation and protection of nervous tissues. To clarify their function in the nervous system in vivo, double knockout (DKO) mice of GM2/GD2 synthase and GD3 synthase genes were generated and abnormal behaviors were analyzed. Mutant mice exhibited reduced weight and a round shape of the whole brain that progressively emerged with aging, and displayed motor dysfunction in the footprint, traction, open-field, and 24 h locomotion activity tests. Sensory functions were also reduced in the von Frey and hot plate tests and greatly reduced in the acoustic startle response test. For emotional behavior, fear response was clearly decreased. Numerous neuronal dysfunctions were found even in younger mutant mice examined at 10-23 weeks after birth, which were exacerbated with aging. These results suggest that a lack of gangliosides other than GM3 induces severe neuronal degeneration in the early stage of life, and that the expression of complex gangliosides is essential to maintain the integrity of the nervous system throughout life.",
author = "Orie Tajima and Nobuaki Egashira and Yuhsuke Ohmi and Yoshihiko Fukue and Kenichi Mishima and Katsunori Iwasaki and Michihiro Fujiwara and Jinichi Inokuchi and Yasuo Sugiura and Keiko Furukawa and Koichi Furukawa",
year = "2009",
month = "3",
day = "2",
doi = "10.1016/j.bbr.2008.10.024",
language = "English",
volume = "198",
pages = "74--82",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Reduced motor and sensory functions and emotional response in GM3-only mice

T2 - Emergence from early stage of life and exacerbation with aging

AU - Tajima, Orie

AU - Egashira, Nobuaki

AU - Ohmi, Yuhsuke

AU - Fukue, Yoshihiko

AU - Mishima, Kenichi

AU - Iwasaki, Katsunori

AU - Fujiwara, Michihiro

AU - Inokuchi, Jinichi

AU - Sugiura, Yasuo

AU - Furukawa, Keiko

AU - Furukawa, Koichi

PY - 2009/3/2

Y1 - 2009/3/2

N2 - Sialic acid-containing glycosphingolipids (gangliosides) have been believed to play a role in the regulation and protection of nervous tissues. To clarify their function in the nervous system in vivo, double knockout (DKO) mice of GM2/GD2 synthase and GD3 synthase genes were generated and abnormal behaviors were analyzed. Mutant mice exhibited reduced weight and a round shape of the whole brain that progressively emerged with aging, and displayed motor dysfunction in the footprint, traction, open-field, and 24 h locomotion activity tests. Sensory functions were also reduced in the von Frey and hot plate tests and greatly reduced in the acoustic startle response test. For emotional behavior, fear response was clearly decreased. Numerous neuronal dysfunctions were found even in younger mutant mice examined at 10-23 weeks after birth, which were exacerbated with aging. These results suggest that a lack of gangliosides other than GM3 induces severe neuronal degeneration in the early stage of life, and that the expression of complex gangliosides is essential to maintain the integrity of the nervous system throughout life.

AB - Sialic acid-containing glycosphingolipids (gangliosides) have been believed to play a role in the regulation and protection of nervous tissues. To clarify their function in the nervous system in vivo, double knockout (DKO) mice of GM2/GD2 synthase and GD3 synthase genes were generated and abnormal behaviors were analyzed. Mutant mice exhibited reduced weight and a round shape of the whole brain that progressively emerged with aging, and displayed motor dysfunction in the footprint, traction, open-field, and 24 h locomotion activity tests. Sensory functions were also reduced in the von Frey and hot plate tests and greatly reduced in the acoustic startle response test. For emotional behavior, fear response was clearly decreased. Numerous neuronal dysfunctions were found even in younger mutant mice examined at 10-23 weeks after birth, which were exacerbated with aging. These results suggest that a lack of gangliosides other than GM3 induces severe neuronal degeneration in the early stage of life, and that the expression of complex gangliosides is essential to maintain the integrity of the nervous system throughout life.

UR - http://www.scopus.com/inward/record.url?scp=58549116492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58549116492&partnerID=8YFLogxK

U2 - 10.1016/j.bbr.2008.10.024

DO - 10.1016/j.bbr.2008.10.024

M3 - Article

C2 - 19013484

AN - SCOPUS:58549116492

VL - 198

SP - 74

EP - 82

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

IS - 1

ER -