Reduced synaptic activity precedes synaptic stripping in vagal motoneurons after axotomy

Jun Yamada, Yoshinori Hayashi, Shozo Jinno, Zhou Wu, Kazuhide Inoue, Shinichi Kohsaka, Hiroshi Nakanishi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Activated microglia, which spread on the motor neurons following nerve injury, engage in the displacement of detached afferent synaptic boutons from the surface of regenerating motor neurons. This phenomenon is known as "synaptic stripping." The present study attempted to examine whether changes in the synaptic inputs after motor nerve injury correlated with the microglial attachment to the dorsal motor neurons of the vagus (DWV). DMV neurons in Wistar rats could survive after nerve injury, whereas most of injured DMV neurons in the C57BL/6 mice died. At 2 days after nerve injury, a significant decrease was observed in the frequencies of both spontaneous and miniature EPSCs and IPSCs recorded from DMV neurons in the slice preparation but not from the mechanically dissociated neurons in the Wistar rats. At this stage, no direct apposition of microglia on the injured neurons was observed. High-K+ stimulation restored their frequencies to control levels. Furthermore, PPADS and DPCPX, antagonists of P2 and adenosine receptors, respectively, also stimulated the recovery of their frequencies. In contrast, no significant change was detected in the spontaneous EPSCs frequency recorded from the severely injured DMV neurons in the slice preparation of the C57BL/6 mice. These observations strongly suggest that presynaptic inhibition through glia-derived ATP and adenosine, thus precedes synaptic stripping in regenerating DMV neurons following nerve injury.

Original languageEnglish
Pages (from-to)1448-1462
Number of pages15
JournalGLIA
Volume56
Issue number13
DOIs
Publication statusPublished - Oct 1 2008

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Axotomy
Motor Neurons
Neurons
Wounds and Injuries
Microglia
Inbred C57BL Mouse
Wistar Rats
Purinergic P1 Receptor Antagonists
Presynaptic Terminals
Neuroglia
Adenosine
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Reduced synaptic activity precedes synaptic stripping in vagal motoneurons after axotomy. / Yamada, Jun; Hayashi, Yoshinori; Jinno, Shozo; Wu, Zhou; Inoue, Kazuhide; Kohsaka, Shinichi; Nakanishi, Hiroshi.

In: GLIA, Vol. 56, No. 13, 01.10.2008, p. 1448-1462.

Research output: Contribution to journalArticle

Yamada, Jun ; Hayashi, Yoshinori ; Jinno, Shozo ; Wu, Zhou ; Inoue, Kazuhide ; Kohsaka, Shinichi ; Nakanishi, Hiroshi. / Reduced synaptic activity precedes synaptic stripping in vagal motoneurons after axotomy. In: GLIA. 2008 ; Vol. 56, No. 13. pp. 1448-1462.
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