Reduction of inorganic phosphate-induced human smooth muscle cells calcification by inhibition of protein kinase A and p38 mitogen-activated protein kinase

Jeong Hun Kang, Riki Toita, Daisuke Asai, Tetsuji Yamaoka, Masaharu Murata

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    High levels of serum phosphate are associated with calcification of human smooth muscle cells (HSMCs). We investigated whether inhibition of protein kinase A (PKA) and mitogen-activated protein kinase (MAPK) signals [p38, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK)] can reduce inorganic phosphate (Pi)-induced HSMC calcification. Inhibition of PKA or p38 MAPK by inhibitors or small interfering RNAs (siRNAs) reduced Ca levels and alkaline phosphatase activities in HSMCs treated with high Pi, but inhibition of ERK1/2 and JNK showed no significant changes. Moreover, there were no significant changes in cell viability on adding siRNAs and three inhibitors (PKA, p38, and MEK1/2), but JNK inhibitor slightly reduced cell viability. These results show that PKA and p38 MAPK are involved in the Pi-induced calcification of HSMCs, and may be good targets for reducing vascular calcification.

    Original languageEnglish
    Pages (from-to)718-722
    Number of pages5
    JournalHeart and Vessels
    Volume29
    Issue number5
    DOIs
    Publication statusPublished - Jan 1 2013

    Fingerprint

    p38 Mitogen-Activated Protein Kinases
    Cyclic AMP-Dependent Protein Kinases
    Smooth Muscle Myocytes
    Mitogen-Activated Protein Kinase 9
    Phosphates
    Small Interfering RNA
    Cell Survival
    Vascular Calcification
    JNK Mitogen-Activated Protein Kinases
    Extracellular Signal-Regulated MAP Kinases
    Protein Kinase Inhibitors
    Human Activities
    Alkaline Phosphatase
    Serum

    All Science Journal Classification (ASJC) codes

    • Cardiology and Cardiovascular Medicine

    Cite this

    Reduction of inorganic phosphate-induced human smooth muscle cells calcification by inhibition of protein kinase A and p38 mitogen-activated protein kinase. / Kang, Jeong Hun; Toita, Riki; Asai, Daisuke; Yamaoka, Tetsuji; Murata, Masaharu.

    In: Heart and Vessels, Vol. 29, No. 5, 01.01.2013, p. 718-722.

    Research output: Contribution to journalArticle

    Kang, Jeong Hun ; Toita, Riki ; Asai, Daisuke ; Yamaoka, Tetsuji ; Murata, Masaharu. / Reduction of inorganic phosphate-induced human smooth muscle cells calcification by inhibition of protein kinase A and p38 mitogen-activated protein kinase. In: Heart and Vessels. 2013 ; Vol. 29, No. 5. pp. 718-722.
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