Reduction of the primary donor P700 of photosystem I during steady-state photosynthesis under low light in Arabidopsis

Michito Tsuyama, Yoshichika Kobayashi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

During steady-state photosynthesis in low-light, 830-nm absorption (A 830) by leaves was close to that in darkness in Arabidopsis, indicating that the primary donor P700 in the reaction center of photosystem I (PSI) was in reduced form. However, P700 was not fully oxidized by a saturating light pulse, suggesting the presence of a population of PSI centers with reduced P700 that remains thermodynamically stable during the application of the saturating light pulse (i.e., reduced-inactive P700). To substantiate this, the effects of methyl viologen (MV) and far-red light on P700 oxidation by the saturating light pulse were analyzed, and the cumulative effects of repetitive application of the saturating light pulse on photosynthesis were analyzed using a mutant crr2-2 with impaired PSI cyclic electron flow. We concluded that the reduced-inactive P700 in low-light as revealed by saturating light pulse indicates limitations of electron flow at the PSI acceptor side.

Original languageEnglish
Pages (from-to)37-47
Number of pages11
JournalPhotosynthesis Research
Volume99
Issue number1
DOIs
Publication statusPublished - Jan 1 2009

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Photosystem I Protein Complex
Photosynthesis
photosystem I
Arabidopsis
photosynthesis
Light
electrons
Electrons
paraquat
far-red light
Paraquat
Darkness
oxidation
mutants
Oxidation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Plant Science
  • Cell Biology

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Reduction of the primary donor P700 of photosystem I during steady-state photosynthesis under low light in Arabidopsis. / Tsuyama, Michito; Kobayashi, Yoshichika.

In: Photosynthesis Research, Vol. 99, No. 1, 01.01.2009, p. 37-47.

Research output: Contribution to journalArticle

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