Abstract
Plastid ω-3 fatty acid desaturase catalyzes the conversion of dienoic fatty acids (16:2 and 18:2) to trienoic fatty acids (16:3 and α-18:3) in glycerolipids which are the main constituents of chloroplast membranes. We produced transgenic tobacco plants that express the transcript of a double-stranded RNA (dsRNA) of tobacco plastid ω-3 fatty acid desaturase gene, NtFAD7. In these transgenic plants, 16:3 and α-18:3 content in leaves decreased to less than 2.7% and 7.5-10.4%, respectively, when compared with the control plant. The steady-state NtFAD7 mRNA was not detected in the transgenic plants. These results indicate that down-regulation of the transcript level in the NtFAD7 by introduction of NtFAD7 dsRNA constructs is useful to decrease the trienoic fatty acid contents of the vegetative tissues in higher plants.
| Original language | English |
|---|---|
| Pages (from-to) | 779-785 |
| Number of pages | 7 |
| Journal | Biotechnology letters |
| Volume | 28 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Jun 1 2006 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Applied Microbiology and Biotechnology
- Microbiology
- Bioengineering
Cite this
Reduction of trienoic fatty acid content by expression of a double-stranded RNA of a plastid ω-3 fatty acid desaturase gene in transgenic tobacco. / Hamada, Tatsuro; Iba, Koh; Shimada, Takiko.
In: Biotechnology letters, Vol. 28, No. 11, 01.06.2006, p. 779-785.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Reduction of trienoic fatty acid content by expression of a double-stranded RNA of a plastid ω-3 fatty acid desaturase gene in transgenic tobacco
AU - Hamada, Tatsuro
AU - Iba, Koh
AU - Shimada, Takiko
PY - 2006/6/1
Y1 - 2006/6/1
N2 - Plastid ω-3 fatty acid desaturase catalyzes the conversion of dienoic fatty acids (16:2 and 18:2) to trienoic fatty acids (16:3 and α-18:3) in glycerolipids which are the main constituents of chloroplast membranes. We produced transgenic tobacco plants that express the transcript of a double-stranded RNA (dsRNA) of tobacco plastid ω-3 fatty acid desaturase gene, NtFAD7. In these transgenic plants, 16:3 and α-18:3 content in leaves decreased to less than 2.7% and 7.5-10.4%, respectively, when compared with the control plant. The steady-state NtFAD7 mRNA was not detected in the transgenic plants. These results indicate that down-regulation of the transcript level in the NtFAD7 by introduction of NtFAD7 dsRNA constructs is useful to decrease the trienoic fatty acid contents of the vegetative tissues in higher plants.
AB - Plastid ω-3 fatty acid desaturase catalyzes the conversion of dienoic fatty acids (16:2 and 18:2) to trienoic fatty acids (16:3 and α-18:3) in glycerolipids which are the main constituents of chloroplast membranes. We produced transgenic tobacco plants that express the transcript of a double-stranded RNA (dsRNA) of tobacco plastid ω-3 fatty acid desaturase gene, NtFAD7. In these transgenic plants, 16:3 and α-18:3 content in leaves decreased to less than 2.7% and 7.5-10.4%, respectively, when compared with the control plant. The steady-state NtFAD7 mRNA was not detected in the transgenic plants. These results indicate that down-regulation of the transcript level in the NtFAD7 by introduction of NtFAD7 dsRNA constructs is useful to decrease the trienoic fatty acid contents of the vegetative tissues in higher plants.
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U2 - 10.1007/s10529-006-9009-7
DO - 10.1007/s10529-006-9009-7
M3 - Article
C2 - 16786241
AN - SCOPUS:33745266401
VL - 28
SP - 779
EP - 785
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 11
ER -