Reduction of trienoic fatty acid content by expression of a double-stranded RNA of a plastid ω-3 fatty acid desaturase gene in transgenic tobacco

Tatsuro Hamada, Koh Iba, Takiko Shimada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Plastid ω-3 fatty acid desaturase catalyzes the conversion of dienoic fatty acids (16:2 and 18:2) to trienoic fatty acids (16:3 and α-18:3) in glycerolipids which are the main constituents of chloroplast membranes. We produced transgenic tobacco plants that express the transcript of a double-stranded RNA (dsRNA) of tobacco plastid ω-3 fatty acid desaturase gene, NtFAD7. In these transgenic plants, 16:3 and α-18:3 content in leaves decreased to less than 2.7% and 7.5-10.4%, respectively, when compared with the control plant. The steady-state NtFAD7 mRNA was not detected in the transgenic plants. These results indicate that down-regulation of the transcript level in the NtFAD7 by introduction of NtFAD7 dsRNA constructs is useful to decrease the trienoic fatty acid contents of the vegetative tissues in higher plants.

Original languageEnglish
Pages (from-to)779-785
Number of pages7
JournalBiotechnology letters
Volume28
Issue number11
DOIs
Publication statusPublished - Jun 1 2006

Fingerprint

Fatty Acid Desaturases
Plastids
Double-Stranded RNA
Tobacco
Genetically Modified Plants
RNA
Fatty acids
Fatty Acids
Genes
Chloroplasts
Down-Regulation
Tissue
Membranes
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Reduction of trienoic fatty acid content by expression of a double-stranded RNA of a plastid ω-3 fatty acid desaturase gene in transgenic tobacco. / Hamada, Tatsuro; Iba, Koh; Shimada, Takiko.

In: Biotechnology letters, Vol. 28, No. 11, 01.06.2006, p. 779-785.

Research output: Contribution to journalArticle

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