Refolding of denatured carbonic anhydrase B by reversed micelles formulated with nonionic surfactant

Masafumi Sakono; Tatsuo Maruyama; Noriho Kamiya; Masahiro Goto

doi:10.1016/j.bej.2004.01.003

Refolding of denatured carbonic anhydrase B by reversed micelles formulated with nonionic surfactant

Masafumi Sakono, Tatsuo Maruyama, Noriho Kamiya, Masahiro Goto

Applied Chemistry

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

We have succeeded in the efficient refolding of denatured carbonic anhydrase B (CAB) using reversed micelles formulated with nonionic surfactant. The reversed micelles with nonionic tetraethylene glycol dodecyl ether prevented denatured CAB from aggregation in the refolding process, allowing a refolding yield of over 70% in 20 h, while the refolding yield of CAB with AOT/isooctane reversed micelle was only about 5%. These results demonstrated that the reversed micelles-mediated protein refolding technique could be extended to the protein which has strong interaction with AOT by simply altering the type of surfactant.

Original language	English
Pages (from-to)	217-220
Number of pages	4
Journal	Biochemical Engineering Journal
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/j.bej.2004.01.003
Publication status	Published - Jul 29 2004

All Science Journal Classification (ASJC) codes

Biotechnology
Environmental Engineering
Bioengineering
Biomedical Engineering

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10.1016/j.bej.2004.01.003

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abstract = "We have succeeded in the efficient refolding of denatured carbonic anhydrase B (CAB) using reversed micelles formulated with nonionic surfactant. The reversed micelles with nonionic tetraethylene glycol dodecyl ether prevented denatured CAB from aggregation in the refolding process, allowing a refolding yield of over 70% in 20 h, while the refolding yield of CAB with AOT/isooctane reversed micelle was only about 5%. These results demonstrated that the reversed micelles-mediated protein refolding technique could be extended to the protein which has strong interaction with AOT by simply altering the type of surfactant.",

author = "Masafumi Sakono and Tatsuo Maruyama and Noriho Kamiya and Masahiro Goto",

note = "Funding Information: This research was supported by the research project to PREST (JST) and by a Grant-in-Aid for scientific research (no. 13010359) from the Ministry of Education, Science and Culture of Japan.",

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AU - Sakono, Masafumi

AU - Maruyama, Tatsuo

AU - Kamiya, Noriho

AU - Goto, Masahiro

N1 - Funding Information: This research was supported by the research project to PREST (JST) and by a Grant-in-Aid for scientific research (no. 13010359) from the Ministry of Education, Science and Culture of Japan.

PY - 2004/7/29

Y1 - 2004/7/29

N2 - We have succeeded in the efficient refolding of denatured carbonic anhydrase B (CAB) using reversed micelles formulated with nonionic surfactant. The reversed micelles with nonionic tetraethylene glycol dodecyl ether prevented denatured CAB from aggregation in the refolding process, allowing a refolding yield of over 70% in 20 h, while the refolding yield of CAB with AOT/isooctane reversed micelle was only about 5%. These results demonstrated that the reversed micelles-mediated protein refolding technique could be extended to the protein which has strong interaction with AOT by simply altering the type of surfactant.

AB - We have succeeded in the efficient refolding of denatured carbonic anhydrase B (CAB) using reversed micelles formulated with nonionic surfactant. The reversed micelles with nonionic tetraethylene glycol dodecyl ether prevented denatured CAB from aggregation in the refolding process, allowing a refolding yield of over 70% in 20 h, while the refolding yield of CAB with AOT/isooctane reversed micelle was only about 5%. These results demonstrated that the reversed micelles-mediated protein refolding technique could be extended to the protein which has strong interaction with AOT by simply altering the type of surfactant.

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Refolding of denatured carbonic anhydrase B by reversed micelles formulated with nonionic surfactant

Abstract

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