Refolding of denatured carbonic anhydrase B by reversed micelles formulated with nonionic surfactant

Masafumi Sakono, Tatsuo Maruyama, Noriho Kamiya, Masahiro Goto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have succeeded in the efficient refolding of denatured carbonic anhydrase B (CAB) using reversed micelles formulated with nonionic surfactant. The reversed micelles with nonionic tetraethylene glycol dodecyl ether prevented denatured CAB from aggregation in the refolding process, allowing a refolding yield of over 70% in 20 h, while the refolding yield of CAB with AOT/isooctane reversed micelle was only about 5%. These results demonstrated that the reversed micelles-mediated protein refolding technique could be extended to the protein which has strong interaction with AOT by simply altering the type of surfactant.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalBiochemical Engineering Journal
Volume19
Issue number3
DOIs
Publication statusPublished - Jul 29 2004

Fingerprint

Carbonic Anhydrase I
Carbonic anhydrase
Nonionic surfactants
Micelles
Surface-Active Agents
Protein Refolding
Proteins
Glycols
Ether
Ethers
Surface active agents
Agglomeration

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

Cite this

Refolding of denatured carbonic anhydrase B by reversed micelles formulated with nonionic surfactant. / Sakono, Masafumi; Maruyama, Tatsuo; Kamiya, Noriho; Goto, Masahiro.

In: Biochemical Engineering Journal, Vol. 19, No. 3, 29.07.2004, p. 217-220.

Research output: Contribution to journalArticle

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