Regeneration of diaphragm with bio-3D cellular patch

Xiu Ying Zhang, Yusuke Yanagi, Zijing Sheng, Kouji Nagata, Koichi Nakayama, Tomoaki Taguchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Neonates with congenital diaphragmatic hernia often require surgical defect closure with a patch. Alternatives to native diaphragmatic tissue are critically needed for this paediatric surgery. The clinical efficacy of mesh patches is limited by complications associated with residual foreign material and by hernia recurrence. In this study, we used a novel bio-3D printer method to generate large scaffold-free tissue patches composed of human cells. The resulting large tissue constructs had high elasticity and strength. Cellular patches were transplanted into rats with surgically created diaphragmatic defects. Rats survived for over 710 days after implantation of tissue constructs. CT confirmed complete tissue integration of the grafts during rat growth. Histology revealed regeneration of muscle structure, neovascularization, and neuronal networks within the reconstructed diaphragms. Our results demonstrate that created cellular patches are a highly safe and effective therapeutic strategy for repairing diaphragmatic defects, and thus pave the way for a clinical trial.

Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalBiomaterials
Volume167
DOIs
Publication statusPublished - Jun 2018

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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    Zhang, X. Y., Yanagi, Y., Sheng, Z., Nagata, K., Nakayama, K., & Taguchi, T. (2018). Regeneration of diaphragm with bio-3D cellular patch. Biomaterials, 167, 1-14. https://doi.org/10.1016/j.biomaterials.2018.03.012