Regeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model

Krzysztof Marek Mrozik, Naohisa Wada, Victor Marino, Ward Richter, Songtao Shi, Donna L. Wheeler, Stan Gronthos, P. Mark Bartold

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Aim: To investigate the capacity of allogeneic periodontal ligament stem cells (PDLSCs) to regenerate periodontal tissues using an ovine periodontal defect model. Materials & methods: Surgically created zero-wall dehiscence periodontal defects created in Merino sheep were filled with 1 × 10 7 allogeneic PDLSCs attached to Gelfoam®, Gelfoam alone or left untreated. After 4 weeks, histological analysis was performed to assess periodontal regeneration. Results: Allogeneic PDLSCs were well tolerated by recipient animals. The mean area of new alveolar bone was significantly greater in the PDLSC + Gelfoam treatment group compared with the defect-alone group. The PDLSC + Gelfoam and Gelfoam-only treatment groups displayed significantly greater length of new cementum and percentage of cementum regrowth compared with the defect-alone group. New Sharpey's fibers were generally more organized and significantly thicker within the PDLSC + Gelfoam treatment group. The PDLSC + Gelfoam treatment group also showed a trend of increased Sharpey's fiber attachment length compared with the Gelfoam-only and defect-alone groups. Conclusion: These studies support the potential use of allogeneic PDLSC preparations as viable therapies for periodontal regeneration in the clinical setting.

Original languageEnglish
Pages (from-to)711-723
Number of pages13
JournalRegenerative Medicine
Volume8
Issue number6
DOIs
Publication statusPublished - Nov 2013

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Absorbable Gelatin Sponge
Periodontal Ligament
Ligaments
Stem cells
Regeneration
Sheep
Stem Cells
Tissue
Defects
Dental Cementum
Fibers
Bone
Animals
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Embryology

Cite this

Mrozik, K. M., Wada, N., Marino, V., Richter, W., Shi, S., Wheeler, D. L., ... Bartold, P. M. (2013). Regeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model. Regenerative Medicine, 8(6), 711-723. https://doi.org/10.2217/rme.13.66

Regeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model. / Mrozik, Krzysztof Marek; Wada, Naohisa; Marino, Victor; Richter, Ward; Shi, Songtao; Wheeler, Donna L.; Gronthos, Stan; Bartold, P. Mark.

In: Regenerative Medicine, Vol. 8, No. 6, 11.2013, p. 711-723.

Research output: Contribution to journalArticle

Mrozik, KM, Wada, N, Marino, V, Richter, W, Shi, S, Wheeler, DL, Gronthos, S & Bartold, PM 2013, 'Regeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model', Regenerative Medicine, vol. 8, no. 6, pp. 711-723. https://doi.org/10.2217/rme.13.66
Mrozik, Krzysztof Marek ; Wada, Naohisa ; Marino, Victor ; Richter, Ward ; Shi, Songtao ; Wheeler, Donna L. ; Gronthos, Stan ; Bartold, P. Mark. / Regeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model. In: Regenerative Medicine. 2013 ; Vol. 8, No. 6. pp. 711-723.
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