Regional and temporal changes in proteomic profile after middle cerebral artery occlusion with or without reperfusion in rats

Hiroshi Yao, Tatsuo Nakahara, Nobuaki Nakagawa, Kijiro Hashimoto, Toshihide Kuroki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Although DNA microarray studies showed up-regulation of various genes, failures of translation of many genes are expected to occur under ischemic conditions even in the penumbra with mild reduction in cerebral blood flow. We applied surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS) technology to study proteomic profile at 6, 12, and 24 h after photothrombotic middle cerebral artery (MCA) occlusion with or without YAG laser-induced reperfusion in adult male spontaneously hypertensive rats. Of the 43 protein peaks that differed from the sham-operation group with a criterion (no overlap of peak intensities between the two groups), 36 peaks (84%) were down-regulated, and seven were up-regulated. All increased peaks showed greater than twofold increases (up to 8.1 fold) compared with those in the sham-operation group. Effects of reperfusion were observed mainly at 24 h after 1 h of MCA occlusion only in the penumbra, where 23 of 32 peaks returned toward the control values, whereas none of 33 peaks showed such attenuation in the ischemic core. Major ischemia-induced changes in protein peaks detected with SELDI-TOF-MS were down-regulations. The present study showed that dynamic changes of protein profile were associated with progression and recovery of the ischemic core and penumbra.

Original languageEnglish
Pages (from-to)1999-2007
Number of pages9
JournalNeurochemical Research
Volume34
Issue number11
DOIs
Publication statusPublished - May 15 2009
Externally publishedYes

Fingerprint

Middle Cerebral Artery Infarction
Proteomics
Reperfusion
Rats
Ionization
Mass spectrometry
Lasers
Desorption
Mass Spectrometry
Cerebrovascular Circulation
Genes
Proteins
Solid-State Lasers
Inbred SHR Rats
Microarrays
Oligonucleotide Array Sequence Analysis
Blood
Up-Regulation
Down-Regulation
Ischemia

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Regional and temporal changes in proteomic profile after middle cerebral artery occlusion with or without reperfusion in rats. / Yao, Hiroshi; Nakahara, Tatsuo; Nakagawa, Nobuaki; Hashimoto, Kijiro; Kuroki, Toshihide.

In: Neurochemical Research, Vol. 34, No. 11, 15.05.2009, p. 1999-2007.

Research output: Contribution to journalArticle

Yao, Hiroshi ; Nakahara, Tatsuo ; Nakagawa, Nobuaki ; Hashimoto, Kijiro ; Kuroki, Toshihide. / Regional and temporal changes in proteomic profile after middle cerebral artery occlusion with or without reperfusion in rats. In: Neurochemical Research. 2009 ; Vol. 34, No. 11. pp. 1999-2007.
@article{cd75a5ebb5b94ab19a167dc085bea7d6,
title = "Regional and temporal changes in proteomic profile after middle cerebral artery occlusion with or without reperfusion in rats",
abstract = "Although DNA microarray studies showed up-regulation of various genes, failures of translation of many genes are expected to occur under ischemic conditions even in the penumbra with mild reduction in cerebral blood flow. We applied surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS) technology to study proteomic profile at 6, 12, and 24 h after photothrombotic middle cerebral artery (MCA) occlusion with or without YAG laser-induced reperfusion in adult male spontaneously hypertensive rats. Of the 43 protein peaks that differed from the sham-operation group with a criterion (no overlap of peak intensities between the two groups), 36 peaks (84{\%}) were down-regulated, and seven were up-regulated. All increased peaks showed greater than twofold increases (up to 8.1 fold) compared with those in the sham-operation group. Effects of reperfusion were observed mainly at 24 h after 1 h of MCA occlusion only in the penumbra, where 23 of 32 peaks returned toward the control values, whereas none of 33 peaks showed such attenuation in the ischemic core. Major ischemia-induced changes in protein peaks detected with SELDI-TOF-MS were down-regulations. The present study showed that dynamic changes of protein profile were associated with progression and recovery of the ischemic core and penumbra.",
author = "Hiroshi Yao and Tatsuo Nakahara and Nobuaki Nakagawa and Kijiro Hashimoto and Toshihide Kuroki",
year = "2009",
month = "5",
day = "15",
doi = "10.1007/s11064-009-9988-6",
language = "English",
volume = "34",
pages = "1999--2007",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "11",

}

TY - JOUR

T1 - Regional and temporal changes in proteomic profile after middle cerebral artery occlusion with or without reperfusion in rats

AU - Yao, Hiroshi

AU - Nakahara, Tatsuo

AU - Nakagawa, Nobuaki

AU - Hashimoto, Kijiro

AU - Kuroki, Toshihide

PY - 2009/5/15

Y1 - 2009/5/15

N2 - Although DNA microarray studies showed up-regulation of various genes, failures of translation of many genes are expected to occur under ischemic conditions even in the penumbra with mild reduction in cerebral blood flow. We applied surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS) technology to study proteomic profile at 6, 12, and 24 h after photothrombotic middle cerebral artery (MCA) occlusion with or without YAG laser-induced reperfusion in adult male spontaneously hypertensive rats. Of the 43 protein peaks that differed from the sham-operation group with a criterion (no overlap of peak intensities between the two groups), 36 peaks (84%) were down-regulated, and seven were up-regulated. All increased peaks showed greater than twofold increases (up to 8.1 fold) compared with those in the sham-operation group. Effects of reperfusion were observed mainly at 24 h after 1 h of MCA occlusion only in the penumbra, where 23 of 32 peaks returned toward the control values, whereas none of 33 peaks showed such attenuation in the ischemic core. Major ischemia-induced changes in protein peaks detected with SELDI-TOF-MS were down-regulations. The present study showed that dynamic changes of protein profile were associated with progression and recovery of the ischemic core and penumbra.

AB - Although DNA microarray studies showed up-regulation of various genes, failures of translation of many genes are expected to occur under ischemic conditions even in the penumbra with mild reduction in cerebral blood flow. We applied surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS) technology to study proteomic profile at 6, 12, and 24 h after photothrombotic middle cerebral artery (MCA) occlusion with or without YAG laser-induced reperfusion in adult male spontaneously hypertensive rats. Of the 43 protein peaks that differed from the sham-operation group with a criterion (no overlap of peak intensities between the two groups), 36 peaks (84%) were down-regulated, and seven were up-regulated. All increased peaks showed greater than twofold increases (up to 8.1 fold) compared with those in the sham-operation group. Effects of reperfusion were observed mainly at 24 h after 1 h of MCA occlusion only in the penumbra, where 23 of 32 peaks returned toward the control values, whereas none of 33 peaks showed such attenuation in the ischemic core. Major ischemia-induced changes in protein peaks detected with SELDI-TOF-MS were down-regulations. The present study showed that dynamic changes of protein profile were associated with progression and recovery of the ischemic core and penumbra.

UR - http://www.scopus.com/inward/record.url?scp=70349603047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349603047&partnerID=8YFLogxK

U2 - 10.1007/s11064-009-9988-6

DO - 10.1007/s11064-009-9988-6

M3 - Article

VL - 34

SP - 1999

EP - 2007

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 11

ER -