Regional distribution of D-amino acids in the rat brain

H. Homma, Kenji Hamase, J. A. Lee, K. Sakai, Y. Takigawa, T. Fukushima, T. Santa, K. Tashiro, T. Iwatsubo, M. Yoshikawa, K. Imai

Research output: Contribution to journalArticle

Abstract

Regional distribution of D-amino acids in the rat brain was studied by highly sensitive HPLC analytical method. The method includes fluorogenic derivatization of each amino acid, isolation of each amino acid by reverse-phase HPLC, followed by enantiomeric separation with a chiral stationary phase. D-Amino acid contents were determined in the cerebrum, cerebellum, hippocampus, medulla oblongata. pituitary gland and pineal gland. A remarkably high concentration of D-aspartate was found in the pineal gland (approx. 3,5micromol/g wet tissue) and a smaller amount was found in the pituitary gland (approx. 80nmol/g). D-Alanine was present exclusively in the pituitary gland (approx. 26nmol/g), whereas minute levels of D-leucine were found in the pineal gland (approx. 3.4nmol/g) and the hippocampus (approx. 1.6nmol/g). D-Serine was detected in various regions except for the cerebellum and medulla oblongata. No other D-amino acids were found by this method. In addition, we developed polyclonal anti-D-aspartate antibody, with which we immunolocalized D-aspartate within the pineal gland. D-Aspartate was present in the cytoplasm of pinealocyte, the predominant cell type in the gland. D-Aspartate immunoreactivity was more evident in the distal region than in the proximal region of the gland. The pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, suggesting that D-aspartate may have correlation with melatonin secretion.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number3
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

D-Aspartic Acid
aspartic acid
Rats
Brain
pineal body
Pineal Gland
brain
Amino Acids
amino acids
pituitary gland
rats
Pituitary Gland
medulla oblongata
Medulla Oblongata
melatonin
cerebellum
Melatonin
hippocampus
Cerebellum
Hippocampus

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Homma, H., Hamase, K., Lee, J. A., Sakai, K., Takigawa, Y., Fukushima, T., ... Imai, K. (1997). Regional distribution of D-amino acids in the rat brain. FASEB Journal, 11(3).

Regional distribution of D-amino acids in the rat brain. / Homma, H.; Hamase, Kenji; Lee, J. A.; Sakai, K.; Takigawa, Y.; Fukushima, T.; Santa, T.; Tashiro, K.; Iwatsubo, T.; Yoshikawa, M.; Imai, K.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Homma, H, Hamase, K, Lee, JA, Sakai, K, Takigawa, Y, Fukushima, T, Santa, T, Tashiro, K, Iwatsubo, T, Yoshikawa, M & Imai, K 1997, 'Regional distribution of D-amino acids in the rat brain', FASEB Journal, vol. 11, no. 3.
Homma H, Hamase K, Lee JA, Sakai K, Takigawa Y, Fukushima T et al. Regional distribution of D-amino acids in the rat brain. FASEB Journal. 1997;11(3).
Homma, H. ; Hamase, Kenji ; Lee, J. A. ; Sakai, K. ; Takigawa, Y. ; Fukushima, T. ; Santa, T. ; Tashiro, K. ; Iwatsubo, T. ; Yoshikawa, M. ; Imai, K. / Regional distribution of D-amino acids in the rat brain. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
@article{f357331593e94f4e8c38c8f48671f80a,
title = "Regional distribution of D-amino acids in the rat brain",
abstract = "Regional distribution of D-amino acids in the rat brain was studied by highly sensitive HPLC analytical method. The method includes fluorogenic derivatization of each amino acid, isolation of each amino acid by reverse-phase HPLC, followed by enantiomeric separation with a chiral stationary phase. D-Amino acid contents were determined in the cerebrum, cerebellum, hippocampus, medulla oblongata. pituitary gland and pineal gland. A remarkably high concentration of D-aspartate was found in the pineal gland (approx. 3,5micromol/g wet tissue) and a smaller amount was found in the pituitary gland (approx. 80nmol/g). D-Alanine was present exclusively in the pituitary gland (approx. 26nmol/g), whereas minute levels of D-leucine were found in the pineal gland (approx. 3.4nmol/g) and the hippocampus (approx. 1.6nmol/g). D-Serine was detected in various regions except for the cerebellum and medulla oblongata. No other D-amino acids were found by this method. In addition, we developed polyclonal anti-D-aspartate antibody, with which we immunolocalized D-aspartate within the pineal gland. D-Aspartate was present in the cytoplasm of pinealocyte, the predominant cell type in the gland. D-Aspartate immunoreactivity was more evident in the distal region than in the proximal region of the gland. The pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, suggesting that D-aspartate may have correlation with melatonin secretion.",
author = "H. Homma and Kenji Hamase and Lee, {J. A.} and K. Sakai and Y. Takigawa and T. Fukushima and T. Santa and K. Tashiro and T. Iwatsubo and M. Yoshikawa and K. Imai",
year = "1997",
language = "English",
volume = "11",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Regional distribution of D-amino acids in the rat brain

AU - Homma, H.

AU - Hamase, Kenji

AU - Lee, J. A.

AU - Sakai, K.

AU - Takigawa, Y.

AU - Fukushima, T.

AU - Santa, T.

AU - Tashiro, K.

AU - Iwatsubo, T.

AU - Yoshikawa, M.

AU - Imai, K.

PY - 1997

Y1 - 1997

N2 - Regional distribution of D-amino acids in the rat brain was studied by highly sensitive HPLC analytical method. The method includes fluorogenic derivatization of each amino acid, isolation of each amino acid by reverse-phase HPLC, followed by enantiomeric separation with a chiral stationary phase. D-Amino acid contents were determined in the cerebrum, cerebellum, hippocampus, medulla oblongata. pituitary gland and pineal gland. A remarkably high concentration of D-aspartate was found in the pineal gland (approx. 3,5micromol/g wet tissue) and a smaller amount was found in the pituitary gland (approx. 80nmol/g). D-Alanine was present exclusively in the pituitary gland (approx. 26nmol/g), whereas minute levels of D-leucine were found in the pineal gland (approx. 3.4nmol/g) and the hippocampus (approx. 1.6nmol/g). D-Serine was detected in various regions except for the cerebellum and medulla oblongata. No other D-amino acids were found by this method. In addition, we developed polyclonal anti-D-aspartate antibody, with which we immunolocalized D-aspartate within the pineal gland. D-Aspartate was present in the cytoplasm of pinealocyte, the predominant cell type in the gland. D-Aspartate immunoreactivity was more evident in the distal region than in the proximal region of the gland. The pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, suggesting that D-aspartate may have correlation with melatonin secretion.

AB - Regional distribution of D-amino acids in the rat brain was studied by highly sensitive HPLC analytical method. The method includes fluorogenic derivatization of each amino acid, isolation of each amino acid by reverse-phase HPLC, followed by enantiomeric separation with a chiral stationary phase. D-Amino acid contents were determined in the cerebrum, cerebellum, hippocampus, medulla oblongata. pituitary gland and pineal gland. A remarkably high concentration of D-aspartate was found in the pineal gland (approx. 3,5micromol/g wet tissue) and a smaller amount was found in the pituitary gland (approx. 80nmol/g). D-Alanine was present exclusively in the pituitary gland (approx. 26nmol/g), whereas minute levels of D-leucine were found in the pineal gland (approx. 3.4nmol/g) and the hippocampus (approx. 1.6nmol/g). D-Serine was detected in various regions except for the cerebellum and medulla oblongata. No other D-amino acids were found by this method. In addition, we developed polyclonal anti-D-aspartate antibody, with which we immunolocalized D-aspartate within the pineal gland. D-Aspartate was present in the cytoplasm of pinealocyte, the predominant cell type in the gland. D-Aspartate immunoreactivity was more evident in the distal region than in the proximal region of the gland. The pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, suggesting that D-aspartate may have correlation with melatonin secretion.

UR - http://www.scopus.com/inward/record.url?scp=33750150857&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750150857&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750150857

VL - 11

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -