Regioselective reduction of a steroid in a reversed micellar system with enzymatic NADH-regeneration

Hidehiko Hirakawa, Noriho Kamiya, Tsuyoshi Yata, Teruyuki Nagamune

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The regioselective reduction of androstandione to androsterone by 3α-hydroxysteroid dehydrogenase (HSDH) from Pseudomonas testosteroni was coupled to a cofactor (NADH) regeneration system with the oxidation of ethanol by yeast alcohol dehydrogenase (YADH) in sodium dioctyl sulfosuccinate (AOT)/isooctane reversed micelles. Each reaction was dominated by the system's water content (W0 = [H2O]/[AOT]). The catalytic activity of YADH increased monotonically with W0, whereas HSDH showed bell-shaped dependency on W0. Using a reversed micellar system increased stability of both enzymes in comparison with an aqueous system and prolonged NADH-regeneration; namely, a seven-fold increase in the total turnover number of NADH was achieved. These results suggest that the reversed micellar system is a promising choice for conjugate enzymatic reactions with dehydrogenases in a nonaqueous media.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalBiochemical Engineering Journal
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

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3-Hydroxysteroid Dehydrogenases
NAD
Regeneration
Alcohol Dehydrogenase
Steroids
Comamonas testosteroni
Dioctyl Sulfosuccinic Acid
Androsterone
Enzyme Stability
Yeast
Micelles
System stability
Alcohols
Water content
Catalyst activity
Oxidoreductases
Ethanol
Oxidation
Water
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

Cite this

Regioselective reduction of a steroid in a reversed micellar system with enzymatic NADH-regeneration. / Hirakawa, Hidehiko; Kamiya, Noriho; Yata, Tsuyoshi; Nagamune, Teruyuki.

In: Biochemical Engineering Journal, Vol. 16, No. 1, 01.01.2003, p. 35-40.

Research output: Contribution to journalArticle

Hirakawa, Hidehiko ; Kamiya, Noriho ; Yata, Tsuyoshi ; Nagamune, Teruyuki. / Regioselective reduction of a steroid in a reversed micellar system with enzymatic NADH-regeneration. In: Biochemical Engineering Journal. 2003 ; Vol. 16, No. 1. pp. 35-40.
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