Regulation of aldosterone synthase cytochrome P-450 in rat adrenals by angiotensin II and potassium

Hirotaka Shibata, Tadashi Ogishima, Fumiko Mitani, Hiromichi Suzuki, Marohito Murakami, Takao Saruta, Yuzuru Ishimura

Research output: Contribution to journalArticle

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Abstract

Changes in the levels of aldosterone synthase cytochrome P-450, a recently identified enzyme in rat adrenals, were studied in response to the renin-angiotensin system and K stimuli. As examined by an immunoblot technique, the zona glomerulosa mitochondria from rats fed on a low Na-normal K diet (8.6 mmol Na+and 207 mmol K+/kg of diet) or a low Na-high K (0.2 M KC1 in drinking water) diet for 4-10 days con-tained significantly higher amounts of aldosterone synthase cytochrome P-450 than those from rats fed on a normal diet (86 mmol Na+and 207 mmol K+/kg of diet). Activities of the enzyme were also found to increase by about 10-fold on day 10. In concert with these changes, both plasma renin activity and plasma aldosterone concentration increased, indicating that the renin-angiotensin system was activated in these rats. Feeding with a normal Na-high K diet also induced significantly higher levels of both amount and activity of aldosterone synthase cytochrome P-450 together with an elevated serum K concentra-tion on day 4, though they all decreased to near the control level on the following days. On the other hand, when enalapril malate, an angiotensin I-converting enzyme inhibitor, was administered to the low Na-normal K rats, the increases in the amount and activity of the enzyme as well as in plasma aldosterone concen-tration were suppressed altogether. However, the enalapril ad-ministration to the low Na-high K rats suppressed the increases only partially. These results indicate that the aldosterone syn-thase cytochrome P-450 is an ultimate target of the regulation of aldosterone biosynthesis by angiotensin II and K.

Original languageEnglish
Pages (from-to)2534-2539
Number of pages6
JournalEndocrinology
Volume128
Issue number5
DOIs
Publication statusPublished - May 1 1991

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Cytochrome P-450 CYP11B2
Angiotensin II
Potassium
Diet
Aldosterone
Enalapril
Renin-Angiotensin System
Enzymes
Zona Glomerulosa
Angiotensin-Converting Enzyme Inhibitors
Renin
Drinking Water
Cytochrome P-450 Enzyme System
Mitochondria
Serum

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Regulation of aldosterone synthase cytochrome P-450 in rat adrenals by angiotensin II and potassium. / Shibata, Hirotaka; Ogishima, Tadashi; Mitani, Fumiko; Suzuki, Hiromichi; Murakami, Marohito; Saruta, Takao; Ishimura, Yuzuru.

In: Endocrinology, Vol. 128, No. 5, 01.05.1991, p. 2534-2539.

Research output: Contribution to journalArticle

Shibata, H, Ogishima, T, Mitani, F, Suzuki, H, Murakami, M, Saruta, T & Ishimura, Y 1991, 'Regulation of aldosterone synthase cytochrome P-450 in rat adrenals by angiotensin II and potassium', Endocrinology, vol. 128, no. 5, pp. 2534-2539. https://doi.org/10.1210/endo-128-5-2534
Shibata, Hirotaka ; Ogishima, Tadashi ; Mitani, Fumiko ; Suzuki, Hiromichi ; Murakami, Marohito ; Saruta, Takao ; Ishimura, Yuzuru. / Regulation of aldosterone synthase cytochrome P-450 in rat adrenals by angiotensin II and potassium. In: Endocrinology. 1991 ; Vol. 128, No. 5. pp. 2534-2539.
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