Regulation of Bin1 SH3 domain binding by phosphoinositides

Chie Kojima, Ari Hashimoto, Izumi Yabuta, Mayumi Hirose, Shigeru Hashimoto, Yasunori Kanaho, Hideki Sumimoto, Takahisa Ikegami, Hisataka Sabe

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Bin1/M-amphiphysin-II is an amphiphysin-II isoform highly expressed in transverse tubules of adult striated muscle and is implicated in their biogenesis. Bin1 contains a basic unique amino-acid sequence, Exon10, which interacts with certain phosphoinositides such as phosphatidylinositol-4,5- bisphosphate (PI(4,5)P2), to localize to membranes. Here we found that Exon10 also binds to the src homology 3 (SH3) domain of Bin1 itself, and hence blocks the binding of the SH3 domain to its canonical PxxP ligands, including dynamin. This blockage was released by addition of PI(4,5) P 2 in vitro or in cells overexpressing phosphatidylinositol 4-phosphate 5-kinase. The Exon10-binding interface of the Bin1 SH3 domain largely overlapped with its PxxP-binding interface. We also show that the PLCδ pleckstrin homology domain, another PI(4,5)P2-binding module, cannot substitute for Exon10 in Bin1 function in transverse tubule formation, and suggest the importance of the dual biochemical properties of Exon10 in myogenesis. Our results exemplify a novel mechanism of SH3 domain regulation, and suggest that the SH3-mediated protein-protein interactions of Bin1 are regulated by Exon10 so that it may only occur when Bin1 localizes to certain submembrane areas.

Original languageEnglish
Pages (from-to)4413-4422
Number of pages10
JournalEMBO Journal
Volume23
Issue number22
DOIs
Publication statusPublished - Nov 10 2004

Fingerprint

src Homology Domains
Phosphatidylinositols
Dynamins
Programmable logic controllers
Muscle
Protein Isoforms
Proteins
Ligands
Membranes
Amino Acids
Basic Amino Acids
Striated Muscle
Muscle Development
Amino Acid Sequence
amphiphysin
1-phosphatidylinositol-4-phosphate 5-kinase
platelet protein P47

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Kojima, C., Hashimoto, A., Yabuta, I., Hirose, M., Hashimoto, S., Kanaho, Y., ... Sabe, H. (2004). Regulation of Bin1 SH3 domain binding by phosphoinositides. EMBO Journal, 23(22), 4413-4422. https://doi.org/10.1038/sj.emboj.7600442

Regulation of Bin1 SH3 domain binding by phosphoinositides. / Kojima, Chie; Hashimoto, Ari; Yabuta, Izumi; Hirose, Mayumi; Hashimoto, Shigeru; Kanaho, Yasunori; Sumimoto, Hideki; Ikegami, Takahisa; Sabe, Hisataka.

In: EMBO Journal, Vol. 23, No. 22, 10.11.2004, p. 4413-4422.

Research output: Contribution to journalArticle

Kojima, C, Hashimoto, A, Yabuta, I, Hirose, M, Hashimoto, S, Kanaho, Y, Sumimoto, H, Ikegami, T & Sabe, H 2004, 'Regulation of Bin1 SH3 domain binding by phosphoinositides', EMBO Journal, vol. 23, no. 22, pp. 4413-4422. https://doi.org/10.1038/sj.emboj.7600442
Kojima C, Hashimoto A, Yabuta I, Hirose M, Hashimoto S, Kanaho Y et al. Regulation of Bin1 SH3 domain binding by phosphoinositides. EMBO Journal. 2004 Nov 10;23(22):4413-4422. https://doi.org/10.1038/sj.emboj.7600442
Kojima, Chie ; Hashimoto, Ari ; Yabuta, Izumi ; Hirose, Mayumi ; Hashimoto, Shigeru ; Kanaho, Yasunori ; Sumimoto, Hideki ; Ikegami, Takahisa ; Sabe, Hisataka. / Regulation of Bin1 SH3 domain binding by phosphoinositides. In: EMBO Journal. 2004 ; Vol. 23, No. 22. pp. 4413-4422.
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