Regulation of expression of mouse interferon-induced transmembrane protein like gene-3, Ifitm3 (mil-1, fragilis), in germ cells

Satomi S. Tanaka, Go Nagamatsu, Yuko Tokitake, Miyuki Kasa, Patrick P.L. Tam, Yasuhisa Matsui

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Mouse interferon-induced transmembrane protein (IFITM) gene, Ifitm3 (previously known as mil-1 and fragilis), is expressed in primordial germ cells (PGCs), in their precursors, and in germ cells of the fetal gonads (Saitou et al. [2002] Nature 418:293-300; Tanaka and Matsui [2002] Mech Dev 119S:S261-S267). By examining the expression of green fluorescent protein transgene under the control of DNA sequences flanking exon 1, we have identified domains that direct Ifitm3 transcription in PGCs and their precursors in gastrula stage and 13.5 days post coitum embryos. Germ cell-specific expression is achieved by the activity of a consensus element unique to the lfitm genes, which may act to suppress Ifitm3 expression in somatic tissues. The lack of any influence of the interferon-stimulable response elements on transgene expression in the germ-line suggests that interferon-mediated response is not critical for activating Ifitm3.

Original languageEnglish
Pages (from-to)651-659
Number of pages9
JournalDevelopmental Dynamics
Volume230
Issue number4
DOIs
Publication statusPublished - Aug 1 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Developmental Biology

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