Regulation of mitochondrial D-loops by transcription factor A and single-stranded DNA-binding protein

Chihiro Takamatsu, Shuyo Umeda, Takashi Ohsato, Tersuji Ohno, Yoshito Abe, Atsushi Fukuoh, Hideo Shinagawa, Naotaka Hamasaki, Dongchon Kang

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

During replication, mitochondrial DNA (mtDNA) takes on a triple-stranded structure called a D-loop. Although their physiological roles are not understood, D-loops are implicated in replication and transcription of mtDNA. Little is known about the turnover of D-loops. We investigated the effects of mitochondrial transcription factor A (TFAM) and single-stranded DNA-binding protein (mtSSB) on D-loops. In human HeLa cells, TFAM and mtSSB are, respectively, 1700- and 3000-fold more abundant than mtDNA. This level of TFAM is two orders of magnitude higher than reported previously and is sufficient to wrap human mtDNA entirely. TFAM resolves D-loops in vitro if added in similar stoichiometries. mtSSB inhibits the resolution of mtDNA by TFAM but enhances resolution by RecG, a junction-specific helicase from Escherichia coli. Hence, mtSSB functions in both stabilization and resolution. We propose that TFAM and mtSSB are cooperatively involved in stabilizing D-loops and in the maintenance of mtDNA.

Original languageEnglish
Pages (from-to)451-456
Number of pages6
JournalEMBO Reports
Volume3
Issue number5
DOIs
Publication statusPublished - Jan 1 2002

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DNA-Binding Proteins
Mitochondrial DNA
Transcription Factors
Transcription
HeLa Cells
Stoichiometry
Escherichia coli
Stabilization
Maintenance

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Regulation of mitochondrial D-loops by transcription factor A and single-stranded DNA-binding protein. / Takamatsu, Chihiro; Umeda, Shuyo; Ohsato, Takashi; Ohno, Tersuji; Abe, Yoshito; Fukuoh, Atsushi; Shinagawa, Hideo; Hamasaki, Naotaka; Kang, Dongchon.

In: EMBO Reports, Vol. 3, No. 5, 01.01.2002, p. 451-456.

Research output: Contribution to journalArticle

Takamatsu, C, Umeda, S, Ohsato, T, Ohno, T, Abe, Y, Fukuoh, A, Shinagawa, H, Hamasaki, N & Kang, D 2002, 'Regulation of mitochondrial D-loops by transcription factor A and single-stranded DNA-binding protein', EMBO Reports, vol. 3, no. 5, pp. 451-456. https://doi.org/10.1093/embo-reports/kvf099
Takamatsu, Chihiro ; Umeda, Shuyo ; Ohsato, Takashi ; Ohno, Tersuji ; Abe, Yoshito ; Fukuoh, Atsushi ; Shinagawa, Hideo ; Hamasaki, Naotaka ; Kang, Dongchon. / Regulation of mitochondrial D-loops by transcription factor A and single-stranded DNA-binding protein. In: EMBO Reports. 2002 ; Vol. 3, No. 5. pp. 451-456.
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AU - Shinagawa, Hideo

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