Regulation of mitotic recombination between DNA repeats in centromeres

Faria Zafar, Akiko K. Okita, Atsushi T. Onaka, Jie Su, Yasuhiro Katahira, Jun Ichi Nakayama, Tatsuro Takahashi, Hisao Masukata, Takuro Nakagawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Centromeres that are essential for faithful segregation of chromosomes consist of unique DNA repeats in many eukaryotes. Although recombination is under-represented around centromeres during meiosis, little is known about recombination between centromere repeats in mitotic cells. Here, we compared spontaneous recombination that occurs between ade6B/ade6X inverted repeats integrated at centromere 1 (cen1) or at a non-centromeric ura4 locus in fission yeast. Remarkably, distinct mechanisms of homologous recombination (HR) were observed in centromere and non-centromere regions. Rad51-dependent HR that requires Rad51, Rad54 and Rad52 was predominant in the centromere, whereas Rad51-independent HR that requires Rad52 also occurred in the arm region. Crossovers between inverted repeats (i.e. inversions) were underrepresented in the centromere as compared to the arm region. While heterochromatin was dispensable, Mhf1/CENP-S, Mhf2/CENP-X histone-fold proteins and Fml1/FANCM helicase were required to suppress crossovers. Furthermore, Mhf1 and Fml1 were found to prevent gross chromosomal rearrangements mediated by centromere repeats. These data uncovered the regulation of mitotic recombination between DNA repeats in centromeres and its physiological role in maintaining genome integrity.

Original languageEnglish
Pages (from-to)11222-11235
Number of pages14
JournalNucleic acids research
Volume45
Issue number19
DOIs
Publication statusPublished - Jan 1 2017

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Centromere
Genetic Recombination
DNA
Homologous Recombination
Chromosome Segregation
Heterochromatin
Schizosaccharomyces
Meiosis
Eukaryota
Histones
Genome

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Zafar, F., Okita, A. K., Onaka, A. T., Su, J., Katahira, Y., Nakayama, J. I., ... Nakagawa, T. (2017). Regulation of mitotic recombination between DNA repeats in centromeres. Nucleic acids research, 45(19), 11222-11235. https://doi.org/10.1093/nar/gkx763

Regulation of mitotic recombination between DNA repeats in centromeres. / Zafar, Faria; Okita, Akiko K.; Onaka, Atsushi T.; Su, Jie; Katahira, Yasuhiro; Nakayama, Jun Ichi; Takahashi, Tatsuro; Masukata, Hisao; Nakagawa, Takuro.

In: Nucleic acids research, Vol. 45, No. 19, 01.01.2017, p. 11222-11235.

Research output: Contribution to journalArticle

Zafar, F, Okita, AK, Onaka, AT, Su, J, Katahira, Y, Nakayama, JI, Takahashi, T, Masukata, H & Nakagawa, T 2017, 'Regulation of mitotic recombination between DNA repeats in centromeres', Nucleic acids research, vol. 45, no. 19, pp. 11222-11235. https://doi.org/10.1093/nar/gkx763
Zafar F, Okita AK, Onaka AT, Su J, Katahira Y, Nakayama JI et al. Regulation of mitotic recombination between DNA repeats in centromeres. Nucleic acids research. 2017 Jan 1;45(19):11222-11235. https://doi.org/10.1093/nar/gkx763
Zafar, Faria ; Okita, Akiko K. ; Onaka, Atsushi T. ; Su, Jie ; Katahira, Yasuhiro ; Nakayama, Jun Ichi ; Takahashi, Tatsuro ; Masukata, Hisao ; Nakagawa, Takuro. / Regulation of mitotic recombination between DNA repeats in centromeres. In: Nucleic acids research. 2017 ; Vol. 45, No. 19. pp. 11222-11235.
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