Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum

Ichiko Miyamoto, Jie Liu, Kuniyoshi Shimizu, Masao Sato, Akiko Kukita, Toshio Kukita, Ryuichiro Kondo

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Abstract

The preventative effects of the ethanol extracts of Ganoderma lucidum against the ovariectomized (Ovx)-induced deterioration of bone density in 11-week-old female Sprague Dawley (SD) rats were investigated. The results showed that the G. lucidum-treated Ovx rats showed improved bone density compared with the Ovx rats. We studied the effects of G. lucidum on osteoclastic differentiation using bone marrow cells and RAW 264 cell D-clone (RAW-D). Differentiation, in response to receptor activator of NF-κB ligand (RANKL) and a tumor necrosis factor α (TNF-α), was inhibited by the ethanol extracts of G. lucidum and ganoderic acid DM which was isolated as one of the active compounds by bioassay-guided fractionation. Ganoderic acid DM especially suppresses the expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1). This suppression leads to the inhibition of dendritic cell-specific transmembrane protein (DC-STAMP) expression and reduces osteoclast fusion.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalEuropean Journal of Pharmacology
Volume602
Issue number1
DOIs
Publication statusPublished - Jan 5 2009

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Reishi
Osteogenesis
Bone Density
Ethanol
NFATC Transcription Factors
Osteoclasts
Bone Marrow Cells
Biological Assay
Dendritic Cells
Sprague Dawley Rats
Clone Cells
Tumor Necrosis Factor-alpha
Ligands
3,7-dioxolanosta-8,24-dien-26-oic acid
Proteins

All Science Journal Classification (ASJC) codes

  • Pharmacology

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Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum. / Miyamoto, Ichiko; Liu, Jie; Shimizu, Kuniyoshi; Sato, Masao; Kukita, Akiko; Kukita, Toshio; Kondo, Ryuichiro.

In: European Journal of Pharmacology, Vol. 602, No. 1, 05.01.2009, p. 1-7.

Research output: Contribution to journalArticle

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AU - Kondo, Ryuichiro

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