Regulation of soybean seed germination through ethylene production in response to reactive oxygen species

Yushi Ishibashi, Yuka Koda, Shao Hui Zheng, Takashi Yuasa, Mari Iwaya-Inoue

Research output: Contribution to journalArticle

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Abstract

Background and AimsDespite their toxicity, reactive oxygen species (ROS) play important roles in plant cell signalling pathways, such as mediating responses to stress or infection and in programmed cell death, at lower levels. Although studies have indicated that hydrogen peroxide (H2O 2) promotes seed germination of several plants such as Arabidopsis, barley, wheat, rice and sunflower, the role of H2O2 in soybean seed germination is not well known. The aim of this study therefore was to investigate the relationships between ROS, plant hormones and soybean seed germination.MethodsAn examination was made of soybean seed germination, the expression of genes related to ethylene biosynthesis, endogenous ethylene contents, and the number and area of cells in the root tip, using N-acetylcysteine, an antioxidant, to counteract the effect of ROS.Key ResultsH2O2 promoted germination, which N-acetylcysteine suppressed, suggesting that ROS are involved in the regulation of soybean germination. H2O2 was produced in the embryonic axis after imbibition. N-Acetylcysteine suppressed the expression of genes related to ethylene biosynthesis and the production of endogenous ethylene. Interestingly, ethephon, which is converted to ethylene, and H2O2 reversed the suppression of seed germination by N-acetylcysteine. Furthermore, morphological analysis revealed that N-acetylcysteine suppressed cell elongation at the root tip, and this suppression was also reversed by ethephon or H 2O2 treatments, as was the case in germination. ConclusionsIn soybean seeds, ROS produced in the embryonic axis after imbibition induce the production of endogenous ethylene, which promotes cell elongation in the root tip. This appears to be how ROS regulate soybean seed germination.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalAnnals of Botany
Volume111
Issue number1
DOIs
Publication statusPublished - Jan 1 2013

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acetylcysteine
ethylene production
reactive oxygen species
seed germination
soybeans
ethylene
root tips
ethephon
imbibition
germination
cell growth
gene expression
plant hormones
Helianthus annuus
hydrogen peroxide
stress response
apoptosis
barley
Arabidopsis
cells

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Regulation of soybean seed germination through ethylene production in response to reactive oxygen species. / Ishibashi, Yushi; Koda, Yuka; Zheng, Shao Hui; Yuasa, Takashi; Iwaya-Inoue, Mari.

In: Annals of Botany, Vol. 111, No. 1, 01.01.2013, p. 95-102.

Research output: Contribution to journalArticle

Ishibashi, Yushi ; Koda, Yuka ; Zheng, Shao Hui ; Yuasa, Takashi ; Iwaya-Inoue, Mari. / Regulation of soybean seed germination through ethylene production in response to reactive oxygen species. In: Annals of Botany. 2013 ; Vol. 111, No. 1. pp. 95-102.
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abstract = "Background and AimsDespite their toxicity, reactive oxygen species (ROS) play important roles in plant cell signalling pathways, such as mediating responses to stress or infection and in programmed cell death, at lower levels. Although studies have indicated that hydrogen peroxide (H2O 2) promotes seed germination of several plants such as Arabidopsis, barley, wheat, rice and sunflower, the role of H2O2 in soybean seed germination is not well known. The aim of this study therefore was to investigate the relationships between ROS, plant hormones and soybean seed germination.MethodsAn examination was made of soybean seed germination, the expression of genes related to ethylene biosynthesis, endogenous ethylene contents, and the number and area of cells in the root tip, using N-acetylcysteine, an antioxidant, to counteract the effect of ROS.Key ResultsH2O2 promoted germination, which N-acetylcysteine suppressed, suggesting that ROS are involved in the regulation of soybean germination. H2O2 was produced in the embryonic axis after imbibition. N-Acetylcysteine suppressed the expression of genes related to ethylene biosynthesis and the production of endogenous ethylene. Interestingly, ethephon, which is converted to ethylene, and H2O2 reversed the suppression of seed germination by N-acetylcysteine. Furthermore, morphological analysis revealed that N-acetylcysteine suppressed cell elongation at the root tip, and this suppression was also reversed by ethephon or H 2O2 treatments, as was the case in germination. ConclusionsIn soybean seeds, ROS produced in the embryonic axis after imbibition induce the production of endogenous ethylene, which promotes cell elongation in the root tip. This appears to be how ROS regulate soybean seed germination.",
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