Regulation of steroidogenesis in transgenic mice and zebrafish

Meng Chun Hu; Evelyn Feng Lin Chiang; Sok Keng Tong; Wayne Lai; Nai Chi Hsu; Leo Chi Kwang Wang; Bon chu Chung

doi:10.1016/S0303-7207(00)00385-3

Regulation of steroidogenesis in transgenic mice and zebrafish

Meng Chun Hu, Evelyn Feng Lin Chiang, Sok Keng Tong, Wayne Lai, Nai Chi Hsu, Leo Chi Kwang Wang, Bon chu Chung

Faculty of Medical Sciences

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Steroid hormones are important physiological regulators in the body. Steroid hormones are mainly synthesized in the adrenal and gonads. Their synthesis is stimulated by pituitary hormones through cAMP as an intracellular mediator. The first and rate-limiting step for steroid biosynthesis is catalyzed by CYP11A1. Important regulatory elements for the control of the CYP11A1 gene expression have been characterized both in vitro and in vivo. The SF-1-binding sites are cis-acting elements controlling the basal and cAMP-stimulated gene expression. Our transgenic mouse studies showed that the 2.3kb promoter contains information controlling developmentally regulated gene expression. Finally, we present our results on the cloning of steroidogenic genes in zebrafish, a new model organism for genetic studies.

Original language	English
Pages (from-to)	9-14
Number of pages	6
Journal	Molecular and Cellular Endocrinology
Volume	171
Issue number	1-2
DOIs	https://doi.org/10.1016/S0303-7207(00)00385-3
Publication status	Published - Jan 22 2001

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Endocrinology

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10.1016/S0303-7207(00)00385-3

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title = "Regulation of steroidogenesis in transgenic mice and zebrafish",

abstract = "Steroid hormones are important physiological regulators in the body. Steroid hormones are mainly synthesized in the adrenal and gonads. Their synthesis is stimulated by pituitary hormones through cAMP as an intracellular mediator. The first and rate-limiting step for steroid biosynthesis is catalyzed by CYP11A1. Important regulatory elements for the control of the CYP11A1 gene expression have been characterized both in vitro and in vivo. The SF-1-binding sites are cis-acting elements controlling the basal and cAMP-stimulated gene expression. Our transgenic mouse studies showed that the 2.3kb promoter contains information controlling developmentally regulated gene expression. Finally, we present our results on the cloning of steroidogenic genes in zebrafish, a new model organism for genetic studies.",

author = "Hu, {Meng Chun} and Chiang, {Evelyn Feng Lin} and Tong, {Sok Keng} and Wayne Lai and Hsu, {Nai Chi} and Wang, {Leo Chi Kwang} and Chung, {Bon chu}",

note = "Funding Information: We would like to thank the transgenic mouse facility at Academia Sinica for generation of the transgenic mouse lines. This work was supported by grants NSC89-2311-B-001-099 from the National Science Council, DOH87-HR-609 from the National Health Research Institutes, and by Academia Sinica, Republic of China. ",

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doi = "10.1016/S0303-7207(00)00385-3",

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T1 - Regulation of steroidogenesis in transgenic mice and zebrafish

AU - Hu, Meng Chun

AU - Chiang, Evelyn Feng Lin

AU - Tong, Sok Keng

AU - Lai, Wayne

AU - Hsu, Nai Chi

AU - Wang, Leo Chi Kwang

AU - Chung, Bon chu

N1 - Funding Information: We would like to thank the transgenic mouse facility at Academia Sinica for generation of the transgenic mouse lines. This work was supported by grants NSC89-2311-B-001-099 from the National Science Council, DOH87-HR-609 from the National Health Research Institutes, and by Academia Sinica, Republic of China.

PY - 2001/1/22

Y1 - 2001/1/22

N2 - Steroid hormones are important physiological regulators in the body. Steroid hormones are mainly synthesized in the adrenal and gonads. Their synthesis is stimulated by pituitary hormones through cAMP as an intracellular mediator. The first and rate-limiting step for steroid biosynthesis is catalyzed by CYP11A1. Important regulatory elements for the control of the CYP11A1 gene expression have been characterized both in vitro and in vivo. The SF-1-binding sites are cis-acting elements controlling the basal and cAMP-stimulated gene expression. Our transgenic mouse studies showed that the 2.3kb promoter contains information controlling developmentally regulated gene expression. Finally, we present our results on the cloning of steroidogenic genes in zebrafish, a new model organism for genetic studies.

AB - Steroid hormones are important physiological regulators in the body. Steroid hormones are mainly synthesized in the adrenal and gonads. Their synthesis is stimulated by pituitary hormones through cAMP as an intracellular mediator. The first and rate-limiting step for steroid biosynthesis is catalyzed by CYP11A1. Important regulatory elements for the control of the CYP11A1 gene expression have been characterized both in vitro and in vivo. The SF-1-binding sites are cis-acting elements controlling the basal and cAMP-stimulated gene expression. Our transgenic mouse studies showed that the 2.3kb promoter contains information controlling developmentally regulated gene expression. Finally, we present our results on the cloning of steroidogenic genes in zebrafish, a new model organism for genetic studies.

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Regulation of steroidogenesis in transgenic mice and zebrafish

Abstract

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