Regulation of the cyanobacterial circadian clock by electrochemically controlled extracellular electron transfer

Yue Lu, Koichi Nishio, Shoichi Matsuda, Yuki Toshima, Hiroshi Ito, Tomohiro Konno, Kazuhiko Ishihara, Souichiro Kato, Kazuhito Hashimoto, Shuji Nakanishi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

There is growing awareness that circadian clocks are closely related to the intracellular redox state across a range of species. As the redox state is determined by the exchange of the redox species, electrochemically controlled extracellular electron transfer (EC-EET), a process in which intracellular electrons are exchanged with extracellular electrodes, is a promising approach for the external regulation of circadian clocks. Herein, we discuss whether the circadian clock can be regulated by EC-EET using the cyanobacterium Synechococcus elongatus PCC7942 as a model system. Invivo monitoring of chlorophyll fluorescence revealed that the redox state of the plastoquionone pool could be controlled with EC-EET by simply changing the electrode potential. As a result, the endogenous circadian clock of S. elongatus cells was successfully entrained through periodically modulated EC-EET by emulating the natural light/dark cycle, even under constant illumination conditions. This is the first example of regulating the biological clock by electrochemistry.

Original languageEnglish
Pages (from-to)2208-2211
Number of pages4
JournalAngewandte Chemie - International Edition
Volume53
Issue number8
DOIs
Publication statusPublished - Feb 17 2014

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Circadian Clocks
Clocks
Oxidation-Reduction
Electrons
Electrodes
Biological Clocks
Synechococcus
Electrochemistry
Photoperiod
Cyanobacteria
Chlorophyll
Lighting
Fluorescence
Monitoring

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Catalysis
  • Medicine(all)

Cite this

Regulation of the cyanobacterial circadian clock by electrochemically controlled extracellular electron transfer. / Lu, Yue; Nishio, Koichi; Matsuda, Shoichi; Toshima, Yuki; Ito, Hiroshi; Konno, Tomohiro; Ishihara, Kazuhiko; Kato, Souichiro; Hashimoto, Kazuhito; Nakanishi, Shuji.

In: Angewandte Chemie - International Edition, Vol. 53, No. 8, 17.02.2014, p. 2208-2211.

Research output: Contribution to journalArticle

Lu, Y, Nishio, K, Matsuda, S, Toshima, Y, Ito, H, Konno, T, Ishihara, K, Kato, S, Hashimoto, K & Nakanishi, S 2014, 'Regulation of the cyanobacterial circadian clock by electrochemically controlled extracellular electron transfer', Angewandte Chemie - International Edition, vol. 53, no. 8, pp. 2208-2211. https://doi.org/10.1002/anie.201309560
Lu, Yue ; Nishio, Koichi ; Matsuda, Shoichi ; Toshima, Yuki ; Ito, Hiroshi ; Konno, Tomohiro ; Ishihara, Kazuhiko ; Kato, Souichiro ; Hashimoto, Kazuhito ; Nakanishi, Shuji. / Regulation of the cyanobacterial circadian clock by electrochemically controlled extracellular electron transfer. In: Angewandte Chemie - International Edition. 2014 ; Vol. 53, No. 8. pp. 2208-2211.
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