Abstract
We report that the expression of mRNA and the activity of UDP- glucose:ceramide (Cer) glucosyltransferase-1 (GlcT-1) of human hepatoma Huh7 and mouse melanoma B16 cells increases after treatment with bacterial sphingomyelinase or upon addition of short-chain Cer. Interestingly, however, GlcT-1 gene transcription was not increased by Cer when GlcT-1 cDNA was introduced with the CMV promoter in GlcT-1-deficient GM95 cells, suggesting that the normal promoter region of GlcT-1 gene is essential for the response. The conversion of C6-Cer to C6-GlcCer occurred much more rapidly in GlcT-1- overexpressing Huh7 cells than in mock transfectants. As a result, GlcT-1- overexpressing cells acquired a greater resistance to C6-Cer-mediated cell death. (C) 2000 Federation of European Biochemical Societies.
| Original language | English |
|---|---|
| Pages (from-to) | 247-250 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 475 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 23 2000 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
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Regulation of UDP-glucose:ceramide glucosyltransferase-1 by ceramide. / Komori, Hironobu; Ichikawa, Shinichi; Hirabayashi, Yoshio; Ito, Makoto.
In: FEBS Letters, Vol. 475, No. 3, 23.06.2000, p. 247-250.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of UDP-glucose:ceramide glucosyltransferase-1 by ceramide
AU - Komori, Hironobu
AU - Ichikawa, Shinichi
AU - Hirabayashi, Yoshio
AU - Ito, Makoto
PY - 2000/6/23
Y1 - 2000/6/23
N2 - We report that the expression of mRNA and the activity of UDP- glucose:ceramide (Cer) glucosyltransferase-1 (GlcT-1) of human hepatoma Huh7 and mouse melanoma B16 cells increases after treatment with bacterial sphingomyelinase or upon addition of short-chain Cer. Interestingly, however, GlcT-1 gene transcription was not increased by Cer when GlcT-1 cDNA was introduced with the CMV promoter in GlcT-1-deficient GM95 cells, suggesting that the normal promoter region of GlcT-1 gene is essential for the response. The conversion of C6-Cer to C6-GlcCer occurred much more rapidly in GlcT-1- overexpressing Huh7 cells than in mock transfectants. As a result, GlcT-1- overexpressing cells acquired a greater resistance to C6-Cer-mediated cell death. (C) 2000 Federation of European Biochemical Societies.
AB - We report that the expression of mRNA and the activity of UDP- glucose:ceramide (Cer) glucosyltransferase-1 (GlcT-1) of human hepatoma Huh7 and mouse melanoma B16 cells increases after treatment with bacterial sphingomyelinase or upon addition of short-chain Cer. Interestingly, however, GlcT-1 gene transcription was not increased by Cer when GlcT-1 cDNA was introduced with the CMV promoter in GlcT-1-deficient GM95 cells, suggesting that the normal promoter region of GlcT-1 gene is essential for the response. The conversion of C6-Cer to C6-GlcCer occurred much more rapidly in GlcT-1- overexpressing Huh7 cells than in mock transfectants. As a result, GlcT-1- overexpressing cells acquired a greater resistance to C6-Cer-mediated cell death. (C) 2000 Federation of European Biochemical Societies.
UR - http://www.scopus.com/inward/record.url?scp=0034705815&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034705815&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(00)01696-3
DO - 10.1016/S0014-5793(00)01696-3
M3 - Article
C2 - 10869565
AN - SCOPUS:0034705815
VL - 475
SP - 247
EP - 250
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -