Regulation of UDP-glucose:ceramide glucosyltransferase-1 by ceramide

Hironobu Komori, Shinichi Ichikawa, Yoshio Hirabayashi, Makoto Ito

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We report that the expression of mRNA and the activity of UDP- glucose:ceramide (Cer) glucosyltransferase-1 (GlcT-1) of human hepatoma Huh7 and mouse melanoma B16 cells increases after treatment with bacterial sphingomyelinase or upon addition of short-chain Cer. Interestingly, however, GlcT-1 gene transcription was not increased by Cer when GlcT-1 cDNA was introduced with the CMV promoter in GlcT-1-deficient GM95 cells, suggesting that the normal promoter region of GlcT-1 gene is essential for the response. The conversion of C6-Cer to C6-GlcCer occurred much more rapidly in GlcT-1- overexpressing Huh7 cells than in mock transfectants. As a result, GlcT-1- overexpressing cells acquired a greater resistance to C6-Cer-mediated cell death. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)247-250
Number of pages4
JournalFEBS Letters
Volume475
Issue number3
DOIs
Publication statusPublished - Jun 23 2000

Fingerprint

ceramide glucosyltransferase
Glucosyltransferases
Ceramides
Genes
Sphingomyelin Phosphodiesterase
Experimental Melanomas
Essential Genes
Cell death
Transcription
Genetic Promoter Regions
Hepatocellular Carcinoma
Cell Death
Complementary DNA
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Regulation of UDP-glucose:ceramide glucosyltransferase-1 by ceramide. / Komori, Hironobu; Ichikawa, Shinichi; Hirabayashi, Yoshio; Ito, Makoto.

In: FEBS Letters, Vol. 475, No. 3, 23.06.2000, p. 247-250.

Research output: Contribution to journalArticle

Komori, H, Ichikawa, S, Hirabayashi, Y & Ito, M 2000, 'Regulation of UDP-glucose:ceramide glucosyltransferase-1 by ceramide', FEBS Letters, vol. 475, no. 3, pp. 247-250. https://doi.org/10.1016/S0014-5793(00)01696-3
Komori, Hironobu ; Ichikawa, Shinichi ; Hirabayashi, Yoshio ; Ito, Makoto. / Regulation of UDP-glucose:ceramide glucosyltransferase-1 by ceramide. In: FEBS Letters. 2000 ; Vol. 475, No. 3. pp. 247-250.
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