Regulation of Vav localization in membrane rafts by adaptor molecules Grb2 and BLNK

Sachiko Johmura, Masatsugu Oh-hora, Kazunori Inabe, Yumiko Nishikawa, Katsuhiko Hayashi, Elena Vigorito, Daisuke Kitamura, Martin Turner, Koh Shingu, Masaki Hikida, Tomohiro Kurosaki

    Research output: Contribution to journalArticlepeer-review

    55 Citations (Scopus)

    Abstract

    Despite the importance of the Vav family proteins for B cell receptor (BCR) signaling, their activation mechanisms remain poorly understood. We demonstrate here that adaptor molecules Grb2 and BLNK, in addition to Vav, are required for efficient Rac1 activation in response to BCR stimulation. Loss of either Grb2 or BLNK results in decreased translocation of Vav3 to membrane rafts. By expression of Vav3 as a raft-targeted construct, the defective Rac1 activation in Grb2- or BLNK-deficient B cells is restored. Hence, our findings suggest that Grb2 and BLNK cooperate to localize Vav into membrane rafts, thereby contributing to optimal activation of Vav in B cells.

    Original languageEnglish
    Pages (from-to)777-787
    Number of pages11
    JournalImmunity
    Volume18
    Issue number6
    DOIs
    Publication statusPublished - Jun 1 2003

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology
    • Infectious Diseases

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