Regulation of Vav localization in membrane rafts by adaptor molecules Grb2 and BLNK

Sachiko Johmura, Masatsugu Oh-hora, Kazunori Inabe, Yumiko Nishikawa, Katsuhiko Hayashi, Elena Vigorito, Daisuke Kitamura, Martin Turner, Koh Shingu, Masaki Hikida, Tomohiro Kurosaki

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

Despite the importance of the Vav family proteins for B cell receptor (BCR) signaling, their activation mechanisms remain poorly understood. We demonstrate here that adaptor molecules Grb2 and BLNK, in addition to Vav, are required for efficient Rac1 activation in response to BCR stimulation. Loss of either Grb2 or BLNK results in decreased translocation of Vav3 to membrane rafts. By expression of Vav3 as a raft-targeted construct, the defective Rac1 activation in Grb2- or BLNK-deficient B cells is restored. Hence, our findings suggest that Grb2 and BLNK cooperate to localize Vav into membrane rafts, thereby contributing to optimal activation of Vav in B cells.

Original languageEnglish
Pages (from-to)777-787
Number of pages11
JournalImmunity
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 1 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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