Regulation of very-low-density lipoprotein receptor in hypertrophic rat heart

Hiroaki Masuzaki, Hisato Jingami, Naoki Matsuoka, Osamu Nakagawa, Yoshihiro Ogawa, Megumi Mizuno, Yasunao Yoshimasa, Tokuo Yamamoto, Kazuwa Nakao

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

To elucidate the regulation of very-low-density lipoprotein (VLDL) receptor, we have studied its gene expression in the heart of spontaneously hypertensive rat-stroke prone (SHR-SP, an animal model for hypertension- induced cardiac hypertrophy) compared with Wistar-Kyoto rats. RNase protection assay showed that ventricular VLDL receptor mRNA falls to 41% of normal levels at 4 weeks, when hypertensions is not yet fully developed, and drops further to 14% at 13 weeks, when cardiac hypertrophy is established. Lipoprotein lipase mRNA decreases in parallel with VLDL receptor mRNA. In cultured neonatal rat ventricular cardiomyocytes. VLDL receptor mRNA decreases in parallel with the process of cardiocyte hypertrophy during the 24 hours after treatment with 10-8 mol/L endothelin-1, falling to 40% of the initial value. These results demonstrate that there is downregulation of VLDL receptor gene expression in cardiac hypertrophy both in vivo and in vitro and suggest that the regulation of the VLDL receptor is possibly linked with the switch in energy substrate from lipid to glucose known to occur in cardiac hypertrophy.

Original languageEnglish
Pages (from-to)8-14
Number of pages7
JournalCirculation Research
Volume78
Issue number1
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

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