Regulatory B cells limit CNS inflammation and neurologic deficits in murine experimental stroke

Xuefang Ren, Kozaburo Akiyoshi, Suzan Dziennis, Arthur A. Vandenbark, Paco S. Herson, Patricia D. Hurn, Halina Offner

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Evaluation of infarct volumes and infiltrating immune cell populations in mice after middle cerebral artery occlusion (MCAO) strongly implicates a mixture of both pathogenic and regulatory immune cell subsets in stroke pathogenesis and recovery. Our goal was to evaluate the contribution of B cells to the development of MCAO by comparing infarct volumes and functional outcomes in wild-type (WT) versus B-cell-deficient /xMT mice. The results clearly demonstrate larger infarct volumes, higher mortality, more severe functional deficits, and increased numbers of activated T cells, macrophages, microglial cells, and neutrophils in the affected brain hemisphere of MCAO-treated /xMT versus WT mice. These MCAO-induced changes were completely prevented in B-cell-restored /xMT mice after transfer of highly purified WT GFP + B cells that were detected in the periphery, but not the CNS. In contrast, transfer of B cells from IL-10 mice had no effect on infarct volume when transferred into /xMT mice. These findings strongly support a previously unrecognized activity of IL-10-secreting WT B cells to limit infarct volume, mortality rate, recruitment of inflammatory cells, and functional neurological deficits 48 h after MCAO. Our novel observations are the first to implicate IL-10-secreting B cells as a major regulatory cell type in stroke and suggest that enhancement of regulatory B cells might have application as a novel therapy for this devastating neurologic condition.

Original languageEnglish
Pages (from-to)8556-8563
Number of pages8
JournalJournal of Neuroscience
Volume31
Issue number23
DOIs
Publication statusPublished - Jun 8 2011

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Regulatory B-Lymphocytes
Neurologic Manifestations
Middle Cerebral Artery Infarction
B-Lymphocytes
Stroke
Inflammation
Interleukin-10
Mortality
Nervous System
Neutrophils
Macrophages
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Ren, X., Akiyoshi, K., Dziennis, S., Vandenbark, A. A., Herson, P. S., Hurn, P. D., & Offner, H. (2011). Regulatory B cells limit CNS inflammation and neurologic deficits in murine experimental stroke. Journal of Neuroscience, 31(23), 8556-8563. https://doi.org/10.1523/JNEUROSCI.1623-11.2011

Regulatory B cells limit CNS inflammation and neurologic deficits in murine experimental stroke. / Ren, Xuefang; Akiyoshi, Kozaburo; Dziennis, Suzan; Vandenbark, Arthur A.; Herson, Paco S.; Hurn, Patricia D.; Offner, Halina.

In: Journal of Neuroscience, Vol. 31, No. 23, 08.06.2011, p. 8556-8563.

Research output: Contribution to journalArticle

Ren, X, Akiyoshi, K, Dziennis, S, Vandenbark, AA, Herson, PS, Hurn, PD & Offner, H 2011, 'Regulatory B cells limit CNS inflammation and neurologic deficits in murine experimental stroke', Journal of Neuroscience, vol. 31, no. 23, pp. 8556-8563. https://doi.org/10.1523/JNEUROSCI.1623-11.2011
Ren, Xuefang ; Akiyoshi, Kozaburo ; Dziennis, Suzan ; Vandenbark, Arthur A. ; Herson, Paco S. ; Hurn, Patricia D. ; Offner, Halina. / Regulatory B cells limit CNS inflammation and neurologic deficits in murine experimental stroke. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 23. pp. 8556-8563.
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