Regulatory effects of purine and pyrimidine nucleoside 5'-di(Tri)- phosphate-3'-diphosphates on eucaryote protein synthesis

Jun Ichiro Mukai, Masato Furuichi, Sawao Murao, Toyokazu Nishino

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9 Citations (Scopus)

Abstract

Adenosine-, guanosine-, cytidine- and uridine-5'-di(tri)-phosphate-3'-diphosphates were enzymatically prepared by the use of Streptomyces adephospholyticus ATP nucleotide pyrophospho- kinase (E.C. 2.7.6.4). Their regulatory effects were then investigated on translation of rat liver, rabbit globin and silkworm pupae chorion mRNAs by the wheat germ lysate system in vitro. They were found to exert base-specific and mRNA species-specific stimulatory or inhibitory effects, respectively. In particular, cytidine 5'-diphosphate-3'-diphosphate stimulated chorion mRNA translation 3-fold. The significance and limitations of the findings were discussed in relation to natural occurrence and possible regulatory functions of the nucleotides in eucaryotes.

Original languageEnglish
Pages (from-to)1775-1781
Number of pages7
JournalAgricultural and Biological Chemistry
Volume45
Issue number8
DOIs
Publication statusPublished - Aug 1981

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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