Regulatory Effects of Purine and Pyrimidine Nucleoside 5’-Di(tri)-phosphate-3’-diphosphates on Eucaryote Protein Synthesis

Jun Ichiro Mukai, Masato Furuichi, Sawao Murao, Toyokazu Nishino

Research output: Contribution to journalArticle

Abstract

Adenosine-, guanosine-, cytidine-and ‘-di(tri)-phosphate-3-diphosphates were en-zymatically prepared by the use of Streptomyces adephospholyticus ATP nucleotide pyrophospho-kinase (E.C. 2.7.6.4). Their regulatory effects were then investigated on translation of rat liver, rabbit globin and silkworm pupae chorion mRNAs by the wheat germ lysate system in vitro. They were found to exert base-specific and mRNA species-specific stimulatory or inhibitory effects, respectively. In particular, cytidine 5‘-diphosphate-3’-diphosphate stimulated chorion mRNA translation 3-fold. The significance and limitations of the findings were discussed in relation to natural occurrence and possible regulatory functions of the nucleotides in eucaryotes.

Original languageEnglish
Pages (from-to)1775-1781
Number of pages7
JournalAgricultural and Biological Chemistry
Volume45
Issue number8
DOIs
Publication statusPublished - Jan 1 1981

Fingerprint

pyrimidine nucleosides
Pyrimidine Nucleosides
Purine Nucleosides
cytidine
Chorion
chorion
Diphosphates
purines
translation (genetics)
eukaryotic cells
Nucleotides
protein synthesis
nucleotides
Phosphates
phosphates
Cytidine
guanosine
Pupa
Messenger RNA
Bombyx

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Regulatory Effects of Purine and Pyrimidine Nucleoside 5’-Di(tri)-phosphate-3’-diphosphates on Eucaryote Protein Synthesis. / Mukai, Jun Ichiro; Furuichi, Masato; Murao, Sawao; Nishino, Toyokazu.

In: Agricultural and Biological Chemistry, Vol. 45, No. 8, 01.01.1981, p. 1775-1781.

Research output: Contribution to journalArticle

@article{d163d88b55174d1985da202c0a075ba0,
title = "Regulatory Effects of Purine and Pyrimidine Nucleoside 5’-Di(tri)-phosphate-3’-diphosphates on Eucaryote Protein Synthesis",
abstract = "Adenosine-, guanosine-, cytidine-and ‘-di(tri)-phosphate-3-diphosphates were en-zymatically prepared by the use of Streptomyces adephospholyticus ATP nucleotide pyrophospho-kinase (E.C. 2.7.6.4). Their regulatory effects were then investigated on translation of rat liver, rabbit globin and silkworm pupae chorion mRNAs by the wheat germ lysate system in vitro. They were found to exert base-specific and mRNA species-specific stimulatory or inhibitory effects, respectively. In particular, cytidine 5‘-diphosphate-3’-diphosphate stimulated chorion mRNA translation 3-fold. The significance and limitations of the findings were discussed in relation to natural occurrence and possible regulatory functions of the nucleotides in eucaryotes.",
author = "Mukai, {Jun Ichiro} and Masato Furuichi and Sawao Murao and Toyokazu Nishino",
year = "1981",
month = "1",
day = "1",
doi = "10.1271/bbb1961.45.1775",
language = "English",
volume = "45",
pages = "1775--1781",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "8",

}

TY - JOUR

T1 - Regulatory Effects of Purine and Pyrimidine Nucleoside 5’-Di(tri)-phosphate-3’-diphosphates on Eucaryote Protein Synthesis

AU - Mukai, Jun Ichiro

AU - Furuichi, Masato

AU - Murao, Sawao

AU - Nishino, Toyokazu

PY - 1981/1/1

Y1 - 1981/1/1

N2 - Adenosine-, guanosine-, cytidine-and ‘-di(tri)-phosphate-3-diphosphates were en-zymatically prepared by the use of Streptomyces adephospholyticus ATP nucleotide pyrophospho-kinase (E.C. 2.7.6.4). Their regulatory effects were then investigated on translation of rat liver, rabbit globin and silkworm pupae chorion mRNAs by the wheat germ lysate system in vitro. They were found to exert base-specific and mRNA species-specific stimulatory or inhibitory effects, respectively. In particular, cytidine 5‘-diphosphate-3’-diphosphate stimulated chorion mRNA translation 3-fold. The significance and limitations of the findings were discussed in relation to natural occurrence and possible regulatory functions of the nucleotides in eucaryotes.

AB - Adenosine-, guanosine-, cytidine-and ‘-di(tri)-phosphate-3-diphosphates were en-zymatically prepared by the use of Streptomyces adephospholyticus ATP nucleotide pyrophospho-kinase (E.C. 2.7.6.4). Their regulatory effects were then investigated on translation of rat liver, rabbit globin and silkworm pupae chorion mRNAs by the wheat germ lysate system in vitro. They were found to exert base-specific and mRNA species-specific stimulatory or inhibitory effects, respectively. In particular, cytidine 5‘-diphosphate-3’-diphosphate stimulated chorion mRNA translation 3-fold. The significance and limitations of the findings were discussed in relation to natural occurrence and possible regulatory functions of the nucleotides in eucaryotes.

UR - http://www.scopus.com/inward/record.url?scp=85024319847&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85024319847&partnerID=8YFLogxK

U2 - 10.1271/bbb1961.45.1775

DO - 10.1271/bbb1961.45.1775

M3 - Article

VL - 45

SP - 1775

EP - 1781

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 8

ER -