Regulatory elements for expression of the alkA gene in response to alkylating agents

Masato Furuichi, Cheng Guo Yu, Motoaki Anai, Sakumi Kunihiko, Mutsuo Sekiguchi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Expression of the alkA gene in Escherichia coli is controlled by Ada protein, which binds to a specific region of the alkA promoter and enhances further binding of RNA polymerase holoenzyme to the complex. To determine the sequence recognized by the Ada protein, we introduced various base substitutions into the promoter region of alkA and examined their effects on expression of the gene, both in vivo and in vitro. Base changes within the sequence AAAGCAAA, located between positions -41 and -34 from the transcription initiation site, greatly decreased the frequencies of initiation of transcription. In footprinting experiments, the region containing this sequence was protected by the Ada protein and base changes within this sequence led to failure of binding of Ada protein to the promoter. It is likely that the Ada protein recognizes the AAAGCAAA sequence in the alkA promoter and binds to the region containing the sequence, thereby allowing ready access of RNA polymerase to the promoter. There are considerable differences between the mechanisms of action of Ada protein on the promoters of alkA and ada, even though the expression of both genes is positively regulated by Ada protein.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalMgg Molecular & General Genetics
Volume236
Issue number1
DOIs
Publication statusPublished - Dec 1 1992

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Alkylating Agents
Gene Expression
Proteins
DNA-Directed RNA Polymerases
Genetic Promoter Regions
Holoenzymes
Transcription Initiation Site
Carrier Proteins
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Regulatory elements for expression of the alkA gene in response to alkylating agents. / Furuichi, Masato; Guo Yu, Cheng; Anai, Motoaki; Kunihiko, Sakumi; Sekiguchi, Mutsuo.

In: Mgg Molecular & General Genetics, Vol. 236, No. 1, 01.12.1992, p. 25-32.

Research output: Contribution to journalArticle

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