Regulatory role of γδ T cells in uterine intraepithelial lymphocytes in maternal antifetal immune response

T. Suzuki, K. Hiromatsu, Y. Ando, T. Okamoto, Y. Tomoda, Yasunobu Yoshikai

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    Abstract

    To elucidate the potential roles of the γδ T cells in uterine intraepithelial lymphocytes (IEL) in the regulation of maternal antifetal immune response during pregnancy, we examined the kinetics and function of γδ T cells in uterine IEL obtained from (C3H/He x AKR/J) pregnancy. The number of γδ T cells increased in the uterine IEL in (C3H/HexAKR/J) pregnancy more than those in (C3H/HexC3H/He) pregnancy and much more than in nonpregnant C3H/He mice. The uterine IEL in (C3H/HexC3H/He) pregnancy significantly proliferated in response to AKR/J stimulator cells. In contrast, the uterine IEL in (C3H/HexAKR/J) pregnancy showed little, if any, proliferation in response to the same stimulator cells. γδ T cell depletion from the uterine IEL in (C3H/HexAKR/J) pregnancy restored their responsiveness against AKR/J stimulator cells. Both γδ T cell-enriched fraction and αβ T cell-depleted fraction, but neither γδ T cell-depleted fraction nor αβ T cell-enriched fraction in the uterine IEL exhibited suppressive activity against allogeneic responses of nonpregnant C3H/He LN cells. This suppressive activity was shown by transferring the supernatant of culture medium in the uterine IEL stimulated with AKR/J cells that contained a large amount of TGF-β, and the suppressive activity was significantly blocked by addition of anti-TGF-β mAb to the culture. Taken together, our results suggest that γδ T cells in the uterine IEL suppress the maternal antifetal immune response at the maternal fetal interface at least in part through TGF-β production to prevent a rejection of the fetus.

    Original languageEnglish
    Pages (from-to)4476-4484
    Number of pages9
    JournalJournal of Immunology
    Volume154
    Issue number9
    Publication statusPublished - Jan 1 1995

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    Regulatory T-Lymphocytes
    Mothers
    Lymphocytes
    T-Lymphocytes
    Pregnancy
    Inbred C3H Mouse
    Culture Media
    Fetus

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Suzuki, T., Hiromatsu, K., Ando, Y., Okamoto, T., Tomoda, Y., & Yoshikai, Y. (1995). Regulatory role of γδ T cells in uterine intraepithelial lymphocytes in maternal antifetal immune response. Journal of Immunology, 154(9), 4476-4484.

    Regulatory role of γδ T cells in uterine intraepithelial lymphocytes in maternal antifetal immune response. / Suzuki, T.; Hiromatsu, K.; Ando, Y.; Okamoto, T.; Tomoda, Y.; Yoshikai, Yasunobu.

    In: Journal of Immunology, Vol. 154, No. 9, 01.01.1995, p. 4476-4484.

    Research output: Contribution to journalArticle

    Suzuki, T, Hiromatsu, K, Ando, Y, Okamoto, T, Tomoda, Y & Yoshikai, Y 1995, 'Regulatory role of γδ T cells in uterine intraepithelial lymphocytes in maternal antifetal immune response', Journal of Immunology, vol. 154, no. 9, pp. 4476-4484.
    Suzuki T, Hiromatsu K, Ando Y, Okamoto T, Tomoda Y, Yoshikai Y. Regulatory role of γδ T cells in uterine intraepithelial lymphocytes in maternal antifetal immune response. Journal of Immunology. 1995 Jan 1;154(9):4476-4484.
    Suzuki, T. ; Hiromatsu, K. ; Ando, Y. ; Okamoto, T. ; Tomoda, Y. ; Yoshikai, Yasunobu. / Regulatory role of γδ T cells in uterine intraepithelial lymphocytes in maternal antifetal immune response. In: Journal of Immunology. 1995 ; Vol. 154, No. 9. pp. 4476-4484.
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