Relation between cell-bound exopolysaccharide production via plasmid-encoded genes and rugose colony morphology in the probiotic Lactobacillus brevis KB290

Masanori Fukao, Takeshi Zendo, Takuro Inoue, Nobuo Fuke, Tomoo Moriuchi, Yasuhiro Yamane, Jiro Nakayama, Kenji Sonomoto, Tetsuya Fukaya

Research output: Contribution to journalArticle

Abstract

The probiotic Lactobacillus brevis KB290 is a natural producer of cell-bound exopolysaccharide (EPS), and the plasmid-encoded glycosyltransferase genes are responsible for this EPS production. KB290 forms unique rugose colonies inside an agar medium; this characteristic is useful for detecting and enumerating KB290 in the gut or feces. However, the genetic elements associated with this morphology remain unclear. Here, we aimed to investigate the relation between the plasmid eps genes and rugose colony morphology in KB290. The plasmid-cured mutants formed smooth colonies, and the rugose colony morphology was restored after complementation with the eps genes. The eps genes were successfully cloned and expressed in other L. brevis and L. plantarum strains. In these transformant strains, the presence of the EPS, consisting of glucose and N-acetylglucosamine, correlated with rugose colonies, indicating that EPS is responsible for rugose colony formation. To the best of our knowledge, this is the first report identifying the genetic factors influencing rugose colonies in Lactobacillus strains. This rugose colony formation may serve as a useful selective marker for KB290 in routine laboratory and research settings and can be used to detect the spontaneous loss of plasmids in this strain.

Original languageEnglish
Pages (from-to)1575-1580
Number of pages6
JournalAnimal Science Journal
Volume90
Issue number12
DOIs
Publication statusPublished - Dec 1 2019

Fingerprint

Lactobacillus brevis
exopolysaccharides
Probiotics
probiotics
plasmids
Plasmids
Genes
genes
cells
Glycosyltransferases
glycosyltransferases
N-acetylglucosamine
Acetylglucosamine
Lactobacillus
Feces
Agar
digestive system
agar
feces
Glucose

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Relation between cell-bound exopolysaccharide production via plasmid-encoded genes and rugose colony morphology in the probiotic Lactobacillus brevis KB290. / Fukao, Masanori; Zendo, Takeshi; Inoue, Takuro; Fuke, Nobuo; Moriuchi, Tomoo; Yamane, Yasuhiro; Nakayama, Jiro; Sonomoto, Kenji; Fukaya, Tetsuya.

In: Animal Science Journal, Vol. 90, No. 12, 01.12.2019, p. 1575-1580.

Research output: Contribution to journalArticle

Fukao, Masanori ; Zendo, Takeshi ; Inoue, Takuro ; Fuke, Nobuo ; Moriuchi, Tomoo ; Yamane, Yasuhiro ; Nakayama, Jiro ; Sonomoto, Kenji ; Fukaya, Tetsuya. / Relation between cell-bound exopolysaccharide production via plasmid-encoded genes and rugose colony morphology in the probiotic Lactobacillus brevis KB290. In: Animal Science Journal. 2019 ; Vol. 90, No. 12. pp. 1575-1580.
@article{a6ba069791e34b549c824d26383f3540,
title = "Relation between cell-bound exopolysaccharide production via plasmid-encoded genes and rugose colony morphology in the probiotic Lactobacillus brevis KB290",
abstract = "The probiotic Lactobacillus brevis KB290 is a natural producer of cell-bound exopolysaccharide (EPS), and the plasmid-encoded glycosyltransferase genes are responsible for this EPS production. KB290 forms unique rugose colonies inside an agar medium; this characteristic is useful for detecting and enumerating KB290 in the gut or feces. However, the genetic elements associated with this morphology remain unclear. Here, we aimed to investigate the relation between the plasmid eps genes and rugose colony morphology in KB290. The plasmid-cured mutants formed smooth colonies, and the rugose colony morphology was restored after complementation with the eps genes. The eps genes were successfully cloned and expressed in other L. brevis and L. plantarum strains. In these transformant strains, the presence of the EPS, consisting of glucose and N-acetylglucosamine, correlated with rugose colonies, indicating that EPS is responsible for rugose colony formation. To the best of our knowledge, this is the first report identifying the genetic factors influencing rugose colonies in Lactobacillus strains. This rugose colony formation may serve as a useful selective marker for KB290 in routine laboratory and research settings and can be used to detect the spontaneous loss of plasmids in this strain.",
author = "Masanori Fukao and Takeshi Zendo and Takuro Inoue and Nobuo Fuke and Tomoo Moriuchi and Yasuhiro Yamane and Jiro Nakayama and Kenji Sonomoto and Tetsuya Fukaya",
year = "2019",
month = "12",
day = "1",
doi = "10.1111/asj.13297",
language = "English",
volume = "90",
pages = "1575--1580",
journal = "Animal Science Journal",
issn = "1344-3941",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - Relation between cell-bound exopolysaccharide production via plasmid-encoded genes and rugose colony morphology in the probiotic Lactobacillus brevis KB290

AU - Fukao, Masanori

AU - Zendo, Takeshi

AU - Inoue, Takuro

AU - Fuke, Nobuo

AU - Moriuchi, Tomoo

AU - Yamane, Yasuhiro

AU - Nakayama, Jiro

AU - Sonomoto, Kenji

AU - Fukaya, Tetsuya

PY - 2019/12/1

Y1 - 2019/12/1

N2 - The probiotic Lactobacillus brevis KB290 is a natural producer of cell-bound exopolysaccharide (EPS), and the plasmid-encoded glycosyltransferase genes are responsible for this EPS production. KB290 forms unique rugose colonies inside an agar medium; this characteristic is useful for detecting and enumerating KB290 in the gut or feces. However, the genetic elements associated with this morphology remain unclear. Here, we aimed to investigate the relation between the plasmid eps genes and rugose colony morphology in KB290. The plasmid-cured mutants formed smooth colonies, and the rugose colony morphology was restored after complementation with the eps genes. The eps genes were successfully cloned and expressed in other L. brevis and L. plantarum strains. In these transformant strains, the presence of the EPS, consisting of glucose and N-acetylglucosamine, correlated with rugose colonies, indicating that EPS is responsible for rugose colony formation. To the best of our knowledge, this is the first report identifying the genetic factors influencing rugose colonies in Lactobacillus strains. This rugose colony formation may serve as a useful selective marker for KB290 in routine laboratory and research settings and can be used to detect the spontaneous loss of plasmids in this strain.

AB - The probiotic Lactobacillus brevis KB290 is a natural producer of cell-bound exopolysaccharide (EPS), and the plasmid-encoded glycosyltransferase genes are responsible for this EPS production. KB290 forms unique rugose colonies inside an agar medium; this characteristic is useful for detecting and enumerating KB290 in the gut or feces. However, the genetic elements associated with this morphology remain unclear. Here, we aimed to investigate the relation between the plasmid eps genes and rugose colony morphology in KB290. The plasmid-cured mutants formed smooth colonies, and the rugose colony morphology was restored after complementation with the eps genes. The eps genes were successfully cloned and expressed in other L. brevis and L. plantarum strains. In these transformant strains, the presence of the EPS, consisting of glucose and N-acetylglucosamine, correlated with rugose colonies, indicating that EPS is responsible for rugose colony formation. To the best of our knowledge, this is the first report identifying the genetic factors influencing rugose colonies in Lactobacillus strains. This rugose colony formation may serve as a useful selective marker for KB290 in routine laboratory and research settings and can be used to detect the spontaneous loss of plasmids in this strain.

UR - http://www.scopus.com/inward/record.url?scp=85073945137&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073945137&partnerID=8YFLogxK

U2 - 10.1111/asj.13297

DO - 10.1111/asj.13297

M3 - Article

C2 - 31593351

AN - SCOPUS:85073945137

VL - 90

SP - 1575

EP - 1580

JO - Animal Science Journal

JF - Animal Science Journal

SN - 1344-3941

IS - 12

ER -