Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film

Sadaaki Yamamoto, Masaru Tanaka, Hiroshi Sunami, Keiko Arai, Aiko Takayama, Shigeko Yamashita, Yuka Morita, Masatsugu Shimomura

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Substratum surface morphology plays a vital roles in cellular behavior. Here, we characterized adsorption of fibronectin (Fn) as a typical cell adhesion protein onto honeycomb-patterned films made of poly(ε-caprolactone) (PCL) by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). In order to determine how cells adhere to a honeycomb-patterned film, focal adhesion of cardiac myocytes (CMYs) and endothelial cells (ECs) on the films were studied by using fluorescence labeling of vinculin. Fn adsorbs around the pore edges to form ring-shaped structures. CMYs and ECs adhere onto the honeycomb-patterned films at focal contact points localized around pore edges distributed over the entire cellular surface. The focal contact points on the honeycomb-patterned films correspond well with the adsorption sites of Fn. We suggest that the cell response to honeycomb-patterned films is associated with the adsorption pattern of Fn on the film.

Original languageEnglish
Pages (from-to)3785-3791
Number of pages7
JournalSurface Science
Volume600
Issue number18
DOIs
Publication statusPublished - Sep 15 2006
Externally publishedYes

Fingerprint

Cell adhesion
Fibronectins
adhesion
cells
muscle cells
Endothelial cells
Point contacts
Adsorption
adsorption
Vinculin
porosity
Labeling
marking
Surface morphology
Atomic force microscopy
Microscopic examination
Adhesion
Fluorescence
atomic force microscopy
microscopy

All Science Journal Classification (ASJC) codes

  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Yamamoto, S., Tanaka, M., Sunami, H., Arai, K., Takayama, A., Yamashita, S., ... Shimomura, M. (2006). Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film. Surface Science, 600(18), 3785-3791. https://doi.org/10.1016/j.susc.2006.01.085

Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film. / Yamamoto, Sadaaki; Tanaka, Masaru; Sunami, Hiroshi; Arai, Keiko; Takayama, Aiko; Yamashita, Shigeko; Morita, Yuka; Shimomura, Masatsugu.

In: Surface Science, Vol. 600, No. 18, 15.09.2006, p. 3785-3791.

Research output: Contribution to journalArticle

Yamamoto, S, Tanaka, M, Sunami, H, Arai, K, Takayama, A, Yamashita, S, Morita, Y & Shimomura, M 2006, 'Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film', Surface Science, vol. 600, no. 18, pp. 3785-3791. https://doi.org/10.1016/j.susc.2006.01.085
Yamamoto, Sadaaki ; Tanaka, Masaru ; Sunami, Hiroshi ; Arai, Keiko ; Takayama, Aiko ; Yamashita, Shigeko ; Morita, Yuka ; Shimomura, Masatsugu. / Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film. In: Surface Science. 2006 ; Vol. 600, No. 18. pp. 3785-3791.
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