The interactions of chemokine receptor CCR7 and its ligands are essential for migration of lymphocytes and dendritic cells to lymph nodes. In this study, we found that 4 of 6 (67%) gastric carcinoma cell lines tested expressed functional CCR7 for the chemokine CCL21/6Ckine, as demonstrated by calcium mobilization and actin polymerization assays. Moreover, we also showed that signaling through CCR7 induced chernotactic and invasive responses in CCR7-positive gastric carcinoma cells. In clinical samples, CCR7-positive carcinoma cells were detected by immunohistochemical assay in 42 of 64 (66%) cases, and there was a significant difference in both lymph node metastasis (p<0.001) and lymphatic invasion (p<0.001) between CCR7-positive and -negative cases. Patients with CCR7-positive tumors had a significantly poorer prognosis than those with CCR7-negative tumors (p<0.05). Stepwise regression analysis revealed that the most important factor related to lymph node metastasis was the expression of CCR7. These results indicate that interactions of CCR7 and its ligands are associated with preferential lymph node metastasis of gastric carcinoma.
|Number of pages||7|
|Publication status||Published - Mar 1 2003|
All Science Journal Classification (ASJC) codes
- Cancer Research