Relationship between expression of chemokine receptor CCR7 and lymph node metastasis in gastric carcinoma

Mitsuhiko Ohta, Kohjiro Mashino, Fumiaki Tanaka, Hiroshi Yamaguchi, Michi Sadanaga, Kenji Shibuta, Hiroshi Inoue, Masaki Mori

Research output: Contribution to journalArticle

Abstract

The interactions of chemokine receptor CCR7 and its ligands are essential for migration of lymphocytes and dendritic cells to lymph nodes. In this study, we found that 4 of 6 (67%) gastric carcinoma cell lines tested expressed functional CCR7 for the chemokine CCL21/6Ckine, as demonstrated by calcium mobilization and actin polymerization assays. Moreover, we also showed that signaling through CCR7 induced chernotactic and invasive responses in CCR7-positive gastric carcinoma cells. In clinical samples, CCR7-positive carcinoma cells were detected by immunohistochemical assay in 42 of 64 (66%) cases, and there was a significant difference in both lymph node metastasis (p<0.001) and lymphatic invasion (p<0.001) between CCR7-positive and -negative cases. Patients with CCR7-positive tumors had a significantly poorer prognosis than those with CCR7-negative tumors (p<0.05). Stepwise regression analysis revealed that the most important factor related to lymph node metastasis was the expression of CCR7. These results indicate that interactions of CCR7 and its ligands are associated with preferential lymph node metastasis of gastric carcinoma.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalBiotherapy
Volume17
Issue number2
Publication statusPublished - Mar 1 2003

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Chemokine Receptors
Stomach
Chemokine CCL21
Lymph Nodes
Neoplasm Metastasis
Carcinoma
Ligands
Polymerization
Dendritic Cells
Actins
Neoplasms
Regression Analysis
Lymphocytes
Calcium
Cell Line

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Ohta, M., Mashino, K., Tanaka, F., Yamaguchi, H., Sadanaga, M., Shibuta, K., ... Mori, M. (2003). Relationship between expression of chemokine receptor CCR7 and lymph node metastasis in gastric carcinoma. Biotherapy, 17(2), 139-145.

Relationship between expression of chemokine receptor CCR7 and lymph node metastasis in gastric carcinoma. / Ohta, Mitsuhiko; Mashino, Kohjiro; Tanaka, Fumiaki; Yamaguchi, Hiroshi; Sadanaga, Michi; Shibuta, Kenji; Inoue, Hiroshi; Mori, Masaki.

In: Biotherapy, Vol. 17, No. 2, 01.03.2003, p. 139-145.

Research output: Contribution to journalArticle

Ohta, M, Mashino, K, Tanaka, F, Yamaguchi, H, Sadanaga, M, Shibuta, K, Inoue, H & Mori, M 2003, 'Relationship between expression of chemokine receptor CCR7 and lymph node metastasis in gastric carcinoma', Biotherapy, vol. 17, no. 2, pp. 139-145.
Ohta M, Mashino K, Tanaka F, Yamaguchi H, Sadanaga M, Shibuta K et al. Relationship between expression of chemokine receptor CCR7 and lymph node metastasis in gastric carcinoma. Biotherapy. 2003 Mar 1;17(2):139-145.
Ohta, Mitsuhiko ; Mashino, Kohjiro ; Tanaka, Fumiaki ; Yamaguchi, Hiroshi ; Sadanaga, Michi ; Shibuta, Kenji ; Inoue, Hiroshi ; Mori, Masaki. / Relationship between expression of chemokine receptor CCR7 and lymph node metastasis in gastric carcinoma. In: Biotherapy. 2003 ; Vol. 17, No. 2. pp. 139-145.
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