Release of membrane vesicles containing endotoxic lipopolysaccharide in Escherichia coli O157:H7 clinical isolates

Yuko Meno, Shuji Fujimoto, Kazumi Horikawa, Shin Ichi Yoshida

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Membrane vesicles released by E. coli O157:H7 strains were investigated by immuno-electron microscopy using anti-O157 antibody. Anti-O157 antibody enhanced the negative-staining of vesicles and we found numerous small vesicles clearly formed around bacterial cells. An immunogold-electron microscopic examination confirmed that lipopolysaccharide (LPS) including the O-side chain is present on the surface of the vesicles. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of the purified vesicles showed that the vesicles contained LPS consisting of a lipid-A and an O polysaccharide. In addition, the endotoxic activity of the vesicle was confirmed by a limulus test. These results suggest that the vesicles may play an important role in the pathogenesis of Escherichia coli O157:H7.

Original languageEnglish
Pages (from-to)271-274
Number of pages4
JournalMICROBIOLOGY and IMMUNOLOGY
Volume44
Issue number4
DOIs
Publication statusPublished - Jan 1 2000

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Fingerprint Dive into the research topics of 'Release of membrane vesicles containing endotoxic lipopolysaccharide in Escherichia coli O157:H7 clinical isolates'. Together they form a unique fingerprint.

  • Cite this