Release of membrane vesicles containing endotoxic lipopolysaccharide in Escherichia coli O157:H7 clinical isolates

Yuko Meno, Shuji Fujimoto, Kazumi Horikawa, Shin Ichi Yoshida

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Membrane vesicles released by E. coli O157:H7 strains were investigated by immuno-electron microscopy using anti-O157 antibody. Anti-O157 antibody enhanced the negative-staining of vesicles and we found numerous small vesicles clearly formed around bacterial cells. An immunogold-electron microscopic examination confirmed that lipopolysaccharide (LPS) including the O-side chain is present on the surface of the vesicles. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of the purified vesicles showed that the vesicles contained LPS consisting of a lipid-A and an O polysaccharide. In addition, the endotoxic activity of the vesicle was confirmed by a limulus test. These results suggest that the vesicles may play an important role in the pathogenesis of Escherichia coli O157:H7.

Original languageEnglish
Pages (from-to)271-274
Number of pages4
JournalMICROBIOLOGY and IMMUNOLOGY
Volume44
Issue number4
DOIs
Publication statusPublished - Jan 1 2000

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Escherichia coli O157
Lipopolysaccharides
Anti-Idiotypic Antibodies
Limulus Test
Negative Staining
Lipid A
Membranes
Immunoelectron Microscopy
Sodium Dodecyl Sulfate
Polysaccharides
Polyacrylamide Gel Electrophoresis
Electrons

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Release of membrane vesicles containing endotoxic lipopolysaccharide in Escherichia coli O157:H7 clinical isolates. / Meno, Yuko; Fujimoto, Shuji; Horikawa, Kazumi; Yoshida, Shin Ichi.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 44, No. 4, 01.01.2000, p. 271-274.

Research output: Contribution to journalArticle

Meno, Yuko ; Fujimoto, Shuji ; Horikawa, Kazumi ; Yoshida, Shin Ichi. / Release of membrane vesicles containing endotoxic lipopolysaccharide in Escherichia coli O157:H7 clinical isolates. In: MICROBIOLOGY and IMMUNOLOGY. 2000 ; Vol. 44, No. 4. pp. 271-274.
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