Remarkable thermal stability of doubly intramolecularly cross-linked hen lysozyme

Tadashi Ueda, Kiyonari Masumoto, Ryoji Ishibashi, Takanori So, Taiji Imoto

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In order to examine how a protein can be effectively stabilized, two intramolecular cross-links, Glu35-Trp108 and Lys1-His15, which have few unfavorable interactions in the folded state, were simultaneously introduced into hen lysozyme. Both of the intramolecularly cross-linked lysozymes, 35-108 CL and 1-15 CL, containing crosslinks Glu35-Trp108 and Lys1-His15, respectively, showed increases in thermal stability of 13.9 and 5.2°C, respectively, over that of wild type, at pH 2.7. On the other hand, a doubly cross-linked lysozyme showed an increase in thermal stability of 20.8°C over that of wild type, under identical conditions. Since the sum of the differences in denaturation temperature between wild type and each of the cross-linked lysozymes was nearly equal to that between wild type and the doubly cross-linked lysozyme, we suggest that the efficient stabilization of the lysozyme molecule was the direct result of the double intramolecular cross-links.

Original languageEnglish
Pages (from-to)193-196
Number of pages4
JournalProtein Engineering
Volume13
Issue number3
Publication statusPublished - May 15 2000

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Muramidase
Thermodynamic stability
Enzymes
Hot Temperature
Denaturation
Stabilization
Proteins
Molecules
Temperature

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Ueda, T., Masumoto, K., Ishibashi, R., So, T., & Imoto, T. (2000). Remarkable thermal stability of doubly intramolecularly cross-linked hen lysozyme. Protein Engineering, 13(3), 193-196.

Remarkable thermal stability of doubly intramolecularly cross-linked hen lysozyme. / Ueda, Tadashi; Masumoto, Kiyonari; Ishibashi, Ryoji; So, Takanori; Imoto, Taiji.

In: Protein Engineering, Vol. 13, No. 3, 15.05.2000, p. 193-196.

Research output: Contribution to journalArticle

Ueda, T, Masumoto, K, Ishibashi, R, So, T & Imoto, T 2000, 'Remarkable thermal stability of doubly intramolecularly cross-linked hen lysozyme', Protein Engineering, vol. 13, no. 3, pp. 193-196.
Ueda T, Masumoto K, Ishibashi R, So T, Imoto T. Remarkable thermal stability of doubly intramolecularly cross-linked hen lysozyme. Protein Engineering. 2000 May 15;13(3):193-196.
Ueda, Tadashi ; Masumoto, Kiyonari ; Ishibashi, Ryoji ; So, Takanori ; Imoto, Taiji. / Remarkable thermal stability of doubly intramolecularly cross-linked hen lysozyme. In: Protein Engineering. 2000 ; Vol. 13, No. 3. pp. 193-196.
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