Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Keishiro Isayama, Lijia Zhao, Huatao Chen, Nobuhiko Yamauchi, Yasufumi Shigeyoshi, Seiichi Hashimoto, Masa aki Hattori

Research output: Contribution to journalArticle

Abstract

The rhythmic expression of clock genes in the uterus is attenuated during decidualization. This study focused on Ptgs2, which is essential for decidualization, as a putative clock-controlled gene, and aimed to reveal the functions of clock genes in relation to Ptgs2 during decidualization. We compared the transcript levels of clock genes in the rat uterus on days 4.5 (D4.5) and 6.5 of pregnancy. The transcript levels of clock genes (Per2, Bmal1, Rorα, and Rev-erbα) had decreased at implantation sites on day 6.5 (D6.5e) compared with those on D4.5, whereas Ptgs2 transcripts had increased on D6.5e. Similar observations of Rev-erbα and Ptgs2 were also obtained in the endometrium on D6.5e by immunohistochemistry. In the decidual cells induced by medroxyprogesterone and 2-O-dibutyryl-cAMP, the rhythmic expression levels of clock genes were attenuated, whereas Ptgs2 transcription was induced. These results indicate that decidualization causes the attenuation of clock genes and the induction of Ptgs2. Furthermore, in the experiment of Bmal1 siRNA, the rhythmic expression of clock genes and Ptgs2 was attenuated by the siRNA. Transcript levels of Ptgs2 and prostaglandin (PG)E₂ production were increased by treatment with the Rev-erbα antagonist, suggesting the contribution of the nuclear receptor Rev-erbα to Ptgs2 expression. Moreover, Rev-erbα knockdown enhanced the induction of Ptgs2 transcription and PGE₂ production by forskolin. Chromatin immunoprecipitation-PCR analysis revealed that Rev-erbα could directly bind to a proximal RORE site of Ptgs2. Collectively, this study demonstrates that the attenuation of the circadian clock, especially its core component Rev-erbα, contributes to the induction of Ptgs2 during decidualization.

Original languageEnglish
Pages (from-to)E650-E661
JournalAmerican journal of physiology. Endocrinology and metabolism
Volume308
Issue number8
DOIs
Publication statusPublished - Apr 15 2015

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Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Stromal Cells
Genes
Prostaglandins E
Small Interfering RNA
Uterus
Medroxyprogesterone
Gene Expression
Circadian Clocks
Chromatin Immunoprecipitation
Colforsin
Cytoplasmic and Nuclear Receptors
Endometrium
Immunohistochemistry
Pregnancy
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells. / Isayama, Keishiro; Zhao, Lijia; Chen, Huatao; Yamauchi, Nobuhiko; Shigeyoshi, Yasufumi; Hashimoto, Seiichi; Hattori, Masa aki.

In: American journal of physiology. Endocrinology and metabolism, Vol. 308, No. 8, 15.04.2015, p. E650-E661.

Research output: Contribution to journalArticle

Isayama, Keishiro ; Zhao, Lijia ; Chen, Huatao ; Yamauchi, Nobuhiko ; Shigeyoshi, Yasufumi ; Hashimoto, Seiichi ; Hattori, Masa aki. / Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells. In: American journal of physiology. Endocrinology and metabolism. 2015 ; Vol. 308, No. 8. pp. E650-E661.
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AU - Shigeyoshi, Yasufumi

AU - Hashimoto, Seiichi

AU - Hattori, Masa aki

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