Abstract
Thermal stabilization of the complex between polycytidylic acid [poly(C)] and the modified schizophyllan (SPG) whose hydrophilic side-chain glucose groups are selectively removed utilizing mild Smith-degradation has been investigated. With the decrease in the side-chain glucose groups of schizophyllan, the complex with poly(C) can be considerably stabilized compared with unmodified SPG; for example, the Tm value after the removal of the side-chain glucose groups from 33.3 (unmodified) to 1.0 is enhanced by 14°C. In addition, the thermal stabilization effect is even operative under the physiological conditions ([NaCl] = 0.15 mol dm-3). This effect is exerted owing to the construction of the hydrophobic atmosphere around the complex. Although schizophyllan lost the side-chain glucose groups, it still kept the protection effect of the bound poly(C) chain against RNaseA-mediated hydrolysis as observed for unmodified schizophyllan. The assessment of the cytotoxicity for A375:human malignant melanoma, and HL60:human promyelocytic leukemia revealed that the modified schizophyllan scarcely increases the cytotoxicity. These results indicate that the present modification for schizophyllan is of great significance in a viewpoint to develop the practical gene carriers operative even under the physiological conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 403-411 |
| Number of pages | 9 |
| Journal | Biopolymers |
| Volume | 75 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Dec 5 2004 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Biomaterials
- Organic Chemistry
Cite this
Removal of the side-chain glucose groups from schizophyllan improves the thermal stability of the polycytidylic acid complexes under the physiological conditions. / Koumoto, Kazuya; Karinaga, Ryouji; Mizu, Masami; Anada, Takahisa; Sakurai, Kazuo; Kunitake, Toyoki; Shinkai, Seiji.
In: Biopolymers, Vol. 75, No. 5, 05.12.2004, p. 403-411.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Removal of the side-chain glucose groups from schizophyllan improves the thermal stability of the polycytidylic acid complexes under the physiological conditions
AU - Koumoto, Kazuya
AU - Karinaga, Ryouji
AU - Mizu, Masami
AU - Anada, Takahisa
AU - Sakurai, Kazuo
AU - Kunitake, Toyoki
AU - Shinkai, Seiji
PY - 2004/12/5
Y1 - 2004/12/5
N2 - Thermal stabilization of the complex between polycytidylic acid [poly(C)] and the modified schizophyllan (SPG) whose hydrophilic side-chain glucose groups are selectively removed utilizing mild Smith-degradation has been investigated. With the decrease in the side-chain glucose groups of schizophyllan, the complex with poly(C) can be considerably stabilized compared with unmodified SPG; for example, the Tm value after the removal of the side-chain glucose groups from 33.3 (unmodified) to 1.0 is enhanced by 14°C. In addition, the thermal stabilization effect is even operative under the physiological conditions ([NaCl] = 0.15 mol dm-3). This effect is exerted owing to the construction of the hydrophobic atmosphere around the complex. Although schizophyllan lost the side-chain glucose groups, it still kept the protection effect of the bound poly(C) chain against RNaseA-mediated hydrolysis as observed for unmodified schizophyllan. The assessment of the cytotoxicity for A375:human malignant melanoma, and HL60:human promyelocytic leukemia revealed that the modified schizophyllan scarcely increases the cytotoxicity. These results indicate that the present modification for schizophyllan is of great significance in a viewpoint to develop the practical gene carriers operative even under the physiological conditions.
AB - Thermal stabilization of the complex between polycytidylic acid [poly(C)] and the modified schizophyllan (SPG) whose hydrophilic side-chain glucose groups are selectively removed utilizing mild Smith-degradation has been investigated. With the decrease in the side-chain glucose groups of schizophyllan, the complex with poly(C) can be considerably stabilized compared with unmodified SPG; for example, the Tm value after the removal of the side-chain glucose groups from 33.3 (unmodified) to 1.0 is enhanced by 14°C. In addition, the thermal stabilization effect is even operative under the physiological conditions ([NaCl] = 0.15 mol dm-3). This effect is exerted owing to the construction of the hydrophobic atmosphere around the complex. Although schizophyllan lost the side-chain glucose groups, it still kept the protection effect of the bound poly(C) chain against RNaseA-mediated hydrolysis as observed for unmodified schizophyllan. The assessment of the cytotoxicity for A375:human malignant melanoma, and HL60:human promyelocytic leukemia revealed that the modified schizophyllan scarcely increases the cytotoxicity. These results indicate that the present modification for schizophyllan is of great significance in a viewpoint to develop the practical gene carriers operative even under the physiological conditions.
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UR - http://www.scopus.com/inward/citedby.url?scp=10044242787&partnerID=8YFLogxK
U2 - 10.1002/bip.20148
DO - 10.1002/bip.20148
M3 - Article
C2 - 15457435
AN - SCOPUS:10044242787
VL - 75
SP - 403
EP - 411
JO - Biopolymers
JF - Biopolymers
SN - 0006-3525
IS - 5
ER -